Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201953_lsiu GCTCACCCGCAAGGGGTTCCAGAACAAATCTG(+ACTCAC)GTACCCACGAGGACAAGCCAAACCAAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604201951_zfvq CCTGATCCACTTGGTTTGGCTTGTCCTCGTGG(+ACTCAC)GTACCAGATTTGTTCTGGAACCCCTTGCGGG NGG 10 8 17 8 24 0 100 5 10
    240604201949_chas AAGAGAAGTAGGAGGTTGACTGACCAACTTGG(+ACTCAC)GTACCATAGGATTTTGTTTATTGCATAGGTC NGG 10 8 17 8 24 0 100 5 10
    240604201947_m804 TAGATGACCTATGCAATAAACAAAATCCTATG(+ACTCAC)GTACCCAAGTTGGTCAGTCAACCTCCTACTT NGG 10 8 17 8 24 0 100 5 10
    240604201945_z006 GCTATGCACCCCTCTCCCTGGCACTGACCCTG(+ACTCAC)GATCCCCATCTTCTGTACCCAGTGACTAGAC NGG 10 8 17 8 24 0 100 5 10
    240604201942_d4yu CAGTCGTCTAGTCACTGGGTACAGAAGATGGG(+ACTCAC)GATCCAGGGTCAGTGCCAGGGAGAGGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240604201940_6wrv TTCCTGCCAAACTACCTTGCGAGGTTTCCTGG(+ACTCAC)GTACCCAAGTGGAGCCAAAGCTCACTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240604201938_q6oh GTCCTGTCCTAGTGAGCTTTGGCTCCACTTGG(+ACTCAC)GTACCCAGGAAACCTCGCAAGGTAGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240604201937_78ta CATGGAGAGACTTAATAAAGGGTTTTACAAGG(+ACTCAC)GTACCTGGATCCCAGGGGGAAGTAGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240604201935_9g4a CCAAGGATAAACTACTTCCCCCTGGGATCCAG(+ACTCAC)GTACCCTTGTAAAACCCTTTATTAAGTCTCT NGG 10 8 17 8 24 0 100 5 10