Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201933_yi66 AGACTTAATAAAGGGTTTTACAAGGGTACCTG(+ACTCAC)GATCCCAGGGGGAAGTAGTTTATCCTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604201930_hmvo CTGTGCCCCAAGGATAAACTACTTCCCCCTGG(+ACTCAC)GATCCAGGTACCCTTGTAAAACCCTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240604201929_u8o9 TCTCAAAGATGAGGCCCACATGAACCTGCTGG(+ACTCAC)GATCCCACGAACACATATTGGACCTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604201927_mi2u TCCCTGTGCTCAGGTCCAATATGTGTTCGTGG(+ACTCAC)GATCCCAGCAGGTTCATGTGGGCCTCATCTT NGG 10 8 17 8 24 0 100 5 10
    240604201926_qp3k ACCACATGATAGCTCGCAACCATCTATAATAG(+ACTCAC)GATCCAAACACTCTTCTGGTGTGTCTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240604201924_n2mu AGCTGTCTTCAGACACACCAGAAGAGTGTTTG(+ACTCAC)GATCCTATTATAGATGGTTGCGAGCTATCAT NGG 10 8 17 8 24 0 100 5 10
    240604201923_im6h GTGGCATGCCCAGCCTTCTCTCTGGCCTATTG(+ACTCAC)GTACCCCCCACCCCCAAAAGAAACAAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240604201921_d6sq TTAACCTCCCTTGTTTCTTTTGGGGGTGGGGG(+ACTCAC)GTACCAATAGGCCAGAGAGAAGGCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604201919_l181 TGGGGGCTCTTAAGGATTTCCCAGAAAAACAG(+ACTCAC)GATCCTGGGATGGGACGTGGTTGCTCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240604201917_ivp9 TTTTTTGCCCGAGCAACCACGTCCCATCCCAG(+ACTCAC)GATCCTGTTTTTCTGGGAAATCCTTAAGAGC NGG 10 8 17 8 24 0 100 5 10