Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201915_lo2o ACCACACATACCTTGAGGAGGACTGCCCTAGG(+ACTCAC)GTACCAGGTTTTATTCAGCTCTAAGTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604201913_m5t6 TTACTCTGGGACTTAGAGCTGAATAAAACCTG(+ACTCAC)GTACCCTAGGGCAGTCCTCCTCAAGGTATGT NGG 10 8 17 8 24 0 100 5 10
    240604201912_9bp9 TATGTGTGGGGATCCCTTCACCCACCATACTG(+ACTCAC)GTACCCCTTCTTCAGCTCCATCTGTCCCTAT NGG 10 8 17 8 24 0 100 5 10
    240604201910_al20 AGGGGATAGGGACAGATGGAGCTGAAGAAGGG(+ACTCAC)GTACCAGTATGGTGGGTGAAGGGATCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604201908_uqae CTTCTTCTTTCTCTAGTGCAAGTATGTGTGGG(+ACTCAC)GATCCCTTCACCCACCATACTGGTACCCCTT NGG 10 8 17 8 24 0 100 5 10
    240604201906_axzv TGAAGAAGGGGTACCAGTATGGTGGGTGAAGG(+ACTCAC)GATCCCCACACATACTTGCACTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604201904_jpo3 TATTTGCTGTGCAAAGCACGCCCTAAGATGGG(+ACTCAC)GTACCTTTGATCTCACCGTGCGGCTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604201903_ams7 TTGAATCCTGCAGCCGCACGGTGAGATCAAAG(+ACTCAC)GTACCCCATCTTAGGGCGTGCTTTGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240604201901_cjfa GAAATAGGGAACACCCACATCTTTCTTTCTGG(+ACTCAC)GATCCATGCGGATGACTGTCCTGGTGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240604201900_myr0 CCATCGAAGCCACCAGGACAGTCATCCGCATG(+ACTCAC)GATCCCAGAAAGAAAGATGTGGGTGTTCCCT NGG 10 8 17 8 24 0 100 5 10