Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201825_bqxr TCTGTGGTGCAGACCATAAAAGGAAGCCATTG(+ACTCAC)GTACCTTCTCAGGTACTTAGCTTTTTGGATA NGG 10 8 17 8 24 0 100 5 10
    240604201824_14aq CCTGATATCCAAAAAGCTAAGTACCTGAGAAG(+ACTCAC)GTACCAATGGCTTCCTTTTATGGTCTGCACC NGG 10 8 17 8 24 0 100 5 10
    240604201822_byzw ATGCAGGAGCACCCCTTTACCCATTTTAAAGG(+ACTCAC)GTACCTTGTACATTTATTTAATCCTACTAAT NGG 10 8 17 8 24 0 100 5 10
    240604201821_tg9h TGCACATTAGTAGGATTAAATAAATGTACAAG(+ACTCAC)GTACCCTTTAAAATGGGTAAAGGGGTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240604201819_degc ACTCTGTAGTTGGAAGAGAATAATAGTGCTGG(+ACTCAC)GTACCCCAGAAAGGTACTGCCTGCATTTCAC NGG 10 8 17 8 24 0 100 5 10
    240604201817_kbn6 TTATGGTGAAATGCAGGCAGTACCTTTCTGGG(+ACTCAC)GTACCCAGCACTATTATTCTCTTCCAACTAC NGG 10 8 17 8 24 0 100 5 10
    240604201816_h8ot AAGGGCACTTGGCTCAGTTGGAGACACTTTTG(+ACTCAC)GTACCTGGTTGGGCAAGTGGGGTGGTACTAA NGG 10 8 17 8 24 0 100 5 10
    240604201814_7bgl CGGTATTAGTACCACCCCACTTGCCCAACCAG(+ACTCAC)GTACCAAAAGTGTCTCCAACTGAGCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240604201812_1ral AAGTGGATTGAGTTTTTAGCACCTGAGTGTGG(+ACTCAC)GATCCATTGTCATTTGTTTTCCCAGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240604201811_2j35 AAGTAAAGGTTCTGGGAAAACAAATGACAATG(+ACTCAC)GATCCCACACTCAGGTGCTAAAAACTCAATC NGG 10 8 17 8 24 0 100 5 10