Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201750_4s61 CATATGGGCTATGCAGAGAGATAGGACTTAGG(+ACTCAC)GTACCAGAACTTCCTGCTCTGTGGTCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604201749_hhsn GGATACCTGGGACCACAGAGCAGGAAGTTCTG(+ACTCAC)GTACCCTAAGTCCTATCTCTCTGCATAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604201747_1p1y TTCCCCCTACACTCATTCTGCTTCCCCAGAAG(+ACTCAC)GATCCTAACACAGAAGATCCCACTCTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604201746_0fik TTACGGTGCTAGAGTGGGATCTTCTGTGTTAG(+ACTCAC)GATCCTTCTGGGGAAGCAGAATGAGTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240604201743_ozee TATTGAATTTCTTCAGCAGCCAATGAATCCTG(+ACTCAC)GATCCATACAAAAATATATTTAGCTTCCACC NGG 10 8 17 8 24 0 100 5 10
    240604201742_qz18 TTGCAGGTGGAAGCTAAATATATTTTTGTATG(+ACTCAC)GATCCAGGATTCATTGGCTGCTGAAGAAATT NGG 10 8 17 8 24 0 100 5 10
    240604201740_2aws GAAGATGTTAAGGGAGGGTAGTTAAATCATGG(+ACTCAC)GTACCTAAGCTGGGGTTTTAGATTGAAGATG NGG 10 8 17 8 24 0 100 5 10
    240604201739_8yy5 TTGGGCATCTTCAATCTAAAACCCCAGCTTAG(+ACTCAC)GTACCCATGATTTAACTACCCTCCCTTAACA NGG 10 8 17 8 24 0 100 5 10
    240604201738_opir TTGAGGTATTCGTTGGGGGAGTGATGGAGGAG(+ACTCAC)GATCCAAAATGATTCATTGTATGGATGGCAG NGG 10 8 17 8 24 0 100 5 10
    240604201736_8jr2 CCTTTCTGCCATCCATACAATGAATCATTTTG(+ACTCAC)GATCCTCCTCCATCACTCCCCCAACGAATAC NGG 10 8 17 8 24 0 100 5 10