Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202133_z3cd CTCATTCGCTGAGCCAGTCATGGACTTGGGTG(+ACTCAC)GATCCAGGAGTCAGTTCTGCAGAGAATTGGG NGG 10 8 17 8 24 0 100 5 10
    240604202131_41d7 CTTGGCCCAATTCTCTGCAGAACTGACTCCTG(+ACTCAC)GATCCACCCAAGTCCATGACTGGCTCAGCGA NGG 10 8 17 8 24 0 100 5 10
    240604202129_utdd GATCCAGAGAAGGTGTTGGGACCACTCTGCAG(+ACTCAC)GATCCGGGTCAGCCCGTTGGCCTGAGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240604202127_7mu0 CCCAGGAAGCCTCAGGCCAACGGGCTGACCCG(+ACTCAC)GATCCTGCAGAGTGGTCCCAACACCTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604202126_vu0v GCTGTCTTCAGACACACCAGAAGAGAGCATCG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGTCACCGT NGG 10 8 17 8 24 0 100 5 10
    240604202124_2x4l ACTACACGGTGACTCACAACCATCTGTAATGG(+ACTCAC)GATCCGATGCTCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604202123_ia7l TAGTCCTCAGTCAGGCTAATACAGGAGTCAGG(+ACTCAC)GTACCAGCTCCCTTATACTTTGTTTTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604202121_hljb TACCACCTTCAAAACAAAGTATAAGGGAGCTG(+ACTCAC)GTACCCTGACTCCTGTATTAGCCTGACTGAG NGG 10 8 17 8 24 0 100 5 10
    240604202118_v2yy TGATAACACACAAGTGTACAGCACCCGCCGGG(+ACTCAC)GATCCCAAAGCCTGCTGGTGCTCAGGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240604202117_1m9y TCCAACTGCCCCTGAGCACCAGCAGGCTTTGG(+ACTCAC)GATCCCCGGCGGGTGCTGTACACTTGTGTGT NGG 10 8 17 8 24 0 100 5 10