Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202604_uter ATGTATCCTTACTTTGACAATTAGACACACAG(+ACTCAC)GTACCAGGAAAAAAGGAATTGACTTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240604202602_b0jk TTCCAGCCTGAAAGTCAATTCCTTTTTTCCTG(+ACTCAC)GTACCTGTGTGTCTAATTGTCAAAGTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604202601_9dvu TGGTTTGCATTGACCGCTTTCCTCCTTGACTG(+ACTCAC)GTACCTTTCCCGTCTTTACGTATGGGAATTG NGG 10 8 17 8 24 0 100 5 10
    240604202600_c3cw GAGGGCAATTCCCATACGTAAAGACGGGAAAG(+ACTCAC)GTACCAGTCAAGGAGGAAAGCGGTCAATGCA NGG 10 8 17 8 24 0 100 5 10
    240604202558_in2g GAGCGCATCCCAGCATGGAGCAGGTAGAGTGG(+ACTCAC)GTACCAAGGCACACCCCTAGCTCAGAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240604202555_o1h9 GCCAATAGCCTCTGAGCTAGGGGTGTGCCTTG(+ACTCAC)GTACCCACTCTACCTGCTCCATGCTGGGATG NGG 10 8 17 8 24 0 100 5 10
    240604202554_ikgy CGAGAGGTTTTCACAAACCGAGTCAAAACAAG(+ACTCAC)GTACCAGTTTTGAGCTCACATCAACTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240604202553_lty3 ACACTTAAAAAGTTGATGTGAGCTCAAAACTG(+ACTCAC)GTACCTTGTTTTGACTCGGTTTGTGAAAACC NGG 10 8 17 8 24 0 100 5 10
    240604202551_wg6n TCTCTGTCTTGAGAGACCAGAAGAGAGCATTG(+ACTCAC)GATCCCATTACAGATGATTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604202550_vy3m ACCACATGGTGGCTCACAATCATCTGTAATGG(+ACTCAC)GATCCAATGCTCTCTTCTGGTCTCTCAAGAC NGG 10 8 17 8 24 0 100 5 10