Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202820_8lwi TCACTAAGAGGACTGAAGGATCTAGTTGAGAG(+ACTCAC)GATCCCTCATGAACGTCCACTACCAGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604202818_siz6 TGAGCTCTGGCTGGTAGTGGACGTTCATGAGG(+ACTCAC)GATCCTCTCAACTAGATCCTTCAGTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240604202817_3snq TGGATAGGGTTGGAGCTACTGGGACAGAGCTG(+ACTCAC)GATCCAGCCATGAGTCTTATGTCCTCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604202815_2ui9 TCTGCTCTGGGAGGACATAAGACTCATGGCTG(+ACTCAC)GATCCAGCTCTGTCCCAGTAGCTCCAACCCT NGG 10 8 17 8 24 0 100 5 10
    240604202814_lbd9 GGATCTTCCAGACGCTGAATCGCACGTAGGTG(+ACTCAC)GTACCTACTGTGCTCCTTGGCTGTGCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240604202812_268g TGCTGAGACTGCACAGCCAAGGAGCACAGTAG(+ACTCAC)GTACCACCTACGTGCGATTCAGCGTCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240604202810_3vr8 AAGACCACTTGCTGTTCAAGTTTAAGTCCCAG(+ACTCAC)GATCCACATTGGATGGCTCACAACCGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240604202809_x63w GTCAGACAAGCGGTTGTGAGCCATCCAATGTG(+ACTCAC)GATCCTGGGACTTAAACTTGAACAGCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240604202807_jz7q GCAACCAGGACTCTGAGAGGTTGTGCTGACGG(+ACTCAC)GATCCCTATTTATTATTTTCATCTACTTGGT NGG 10 8 17 8 24 0 100 5 10
    240604202805_w1us TTCGTACCAAGTAGATGAAAATAATAAATAGG(+ACTCAC)GATCCCGTCAGCACAACCTCTCAGAGTCCTG NGG 10 8 17 8 24 0 100 5 10