Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201205_as5d TTGTCATGAGTACTTTACTAAAATATTTCAAG(+ACTCAC)GATCCAAATAGCATCAACTGTTTTCAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604201204_fcnc ATCTGCTTTTTGAAAACAGTTGATGCTATTTG(+ACTCAC)GATCCTTGAAATATTTTAGTAAAGTACTCAT NGG 10 8 17 8 24 0 100 5 10
    240604201203_vxln CAGGGGGCAAATTGGAAGCTGAGATCGACTTG(+ACTCAC)GATCCTGGGAAGGATGAAGATGACCTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240604201200_gl68 CTCAAGCAGAAGGTCATCTTCATCCTTCCCAG(+ACTCAC)GATCCAAGTCGATCTCAGCTTCCAATTTGCC NGG 10 8 17 8 24 0 100 5 10
    240604201158_91f2 AGAACACAGGGGGTCCCCAACCCACTCTCAGG(+ACTCAC)GATCCACCTGGTGTCTTGGGTCTGTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604201157_gcvd TCTGCTCCCTGACAGACCCAAGACACCAGGTG(+ACTCAC)GATCCCTGAGAGTGGGTTGGGGACCCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604201155_llp8 TTCCCCCACCTAACCCACTTTTGAAACCAAAG(+ACTCAC)GTACCTTGCCTCAGCGACACAAAGAGATGGG NGG 10 8 17 8 24 0 100 5 10
    240604201154_guwm GATCTCCCATCTCTTTGTGTCGCTGAGGCAAG(+ACTCAC)GTACCTTTGGTTTCAAAAGTGGGTTAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604201152_d2v1 TCTCTGTCCTGGTCCCGCCCCCAACCATGGTG(+ACTCAC)GATCCTGGAGGACCTCGTACCCCTCCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240604201149_ncpq GTGGGGGAGGGGAGGGGTACGAGGTCCTCCAG(+ACTCAC)GATCCACCATGGTTGGGGGCGGGACCAGGAC NGG 10 8 17 8 24 0 100 5 10