Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201256_0t2i GGCAGTTTCCTGAGACTGTAAAGTATTCCAGG(+ACTCAC)GATCCTTTTCCTGTGCGGATGCAGAAGCCAT NGG 10 8 17 8 24 0 100 5 10
    240604201255_ub1o CTGGCATGGCTTCTGCATCCGCACAGGAAAAG(+ACTCAC)GATCCCTGGAATACTTTACAGTCTCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240604201253_63u7 CAACACCATTTACCTAGAAAGGACCAAGTTTG(+ACTCAC)GATCCTGAAGCCCAGTGGAGCACCATTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604201252_ygd2 CAGGCAGAGAATGGTGCTCCACTGGGCTTCAG(+ACTCAC)GATCCAAACTTGGTCCTTTCTAGGTAAATGG NGG 10 8 17 8 24 0 100 5 10
    240604201250_wasb TGAGAAGAGCCTCTGACAGGCATCAGAGCTGG(+ACTCAC)GATCCTGACCAAGGGACACTCAGGCTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240604201249_v10a AGCTGGTGGCAGCCTGAGTGTCCCTTGGTCAG(+ACTCAC)GATCCCAGCTCTGATGCCTGTCAGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240604201246_0ye1 ACAGATAGTGTATGAAATACACAATTTCCCAG(+ACTCAC)GTACCAAGGGCCAATAGCAAAAAGGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604201244_thne CTTTTAGAGACCCTTTTTGCTATTGGCCCTTG(+ACTCAC)GTACCTGGGAAATTGTGTATTTCATACACTA NGG 10 8 17 8 24 0 100 5 10
    240604201241_s7l8 TTGACTGTCTCTGCATAGTCTTGATACCCCAG(+ACTCAC)GATCCTGACATCCCCAGAAATTATACCCCGC NGG 10 8 17 8 24 0 100 5 10
    240604201239_inlf GGGGGGCGGGGTATAATTTCTGGGGATGTCAG(+ACTCAC)GATCCTGGGGTATCAAGACTATGCAGAGACA NGG 10 8 17 8 24 0 100 5 10