Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201329_xy3r ACCACGGCCTCTGAATGGGGCTGAGGCTCATG(+ACTCAC)GATCCCGGAATGAGACCAGGATGCATAAGAC NGG 10 8 17 8 24 0 100 5 10
    240604201327_4gw8 CACTGGTCTTATGCATCCTGGTCTCATTCCGG(+ACTCAC)GATCCATGAGCCTCAGCCCCATTCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240604201325_c8q7 GGCTTCTTGTGATCTCCTTTGCCAGTCACTGG(+ACTCAC)GATCCAGAACAATCTACTTCTTCAAGCATGC NGG 10 8 17 8 24 0 100 5 10
    240604201324_i2m3 CAATGGCATGCTTGAAGAAGTAGATTGTTCTG(+ACTCAC)GATCCCAGTGACTGGCAAAGGAGATCACAAG NGG 10 8 17 8 24 0 100 5 10
    240604201322_k6n6 GGGTGACTGGATAACCCCAGCATGCCCAGCGG(+ACTCAC)GTACCATAAGCCCACACTGCTGGGATGAGGT NGG 10 8 17 8 24 0 100 5 10
    240604201321_i8yf CTGGGACCTCATCCCAGCAGTGTGGGCTTATG(+ACTCAC)GTACCCGCTGGGCATGCTGGGGTTATCCAGT NGG 10 8 17 8 24 0 100 5 10
    240604201320_3i2v GATGGATAGGATGGATGCACCAACAGCTGTTG(+ACTCAC)GTACCTTGTTTTTCCTTCAACTGTTGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240604201318_3dz5 AGTCAATACCCAACAGTTGAAGGAAAAACAAG(+ACTCAC)GTACCAACAGCTGTTGGTGCATCCATCCTAT NGG 10 8 17 8 24 0 100 5 10
    240604201316_04lz CTCGCATACTTTGACTTCATTTCTATTCCTGG(+ACTCAC)GATCCACCACCCTCTTCTCTCAGTTGACTGC NGG 10 8 17 8 24 0 100 5 10
    240604201314_61h3 ATGCAGCAGTCAACTGAGAGAAGAGGGTGGTG(+ACTCAC)GATCCCAGGAATAGAAATGAAGTCAAAGTAT NGG 10 8 17 8 24 0 100 5 10