Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201417_e05z CTTGCAAAAGCCAAAGCTGCCTGAGTAGTCTG(+ACTCAC)GATCCTGCAACATCACTTTGCTTCAGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604201416_cbt9 AAACTAACTCCTGAAGCAAAGTGATGTTGCAG(+ACTCAC)GATCCAGACTACTCAGGCAGCTTTGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240604201414_dseg CTCCAAAGCTAGAGTAATCATGAGGGAGGCAG(+ACTCAC)GATCCACGGAGAATCACAAAGGATGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240604201412_shty GTATCAAAATGCATCCTTTGTGATTCTCCGTG(+ACTCAC)GATCCTGCCTCCCTCATGATTACTCTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240604201410_ywt6 GTATGCTGAGGTGTGGGGGTGGTGAATGAAGG(+ACTCAC)GTACCCTACCTGCAGTGCTCAAGAATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240604201408_d1w3 ATCATTGGACATTCTTGAGCACTGCAGGTAGG(+ACTCAC)GTACCCTTCATTCACCACCCCCACACCTCAG NGG 10 8 17 8 24 0 100 5 10
    240604201407_vtnl TTAATCCCAGCACTAAGGAGGCAGAGCAGGCG(+ACTCAC)GATCCCAGTGAGTTTGAAGCCTGAGCTATAG NGG 10 8 17 8 24 0 100 5 10
    240604201406_4kxb TCATCCTATAGCTCAGGCTTCAAACTCACTGG(+ACTCAC)GATCCGCCTGCTCTGCCTCCTTAGTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604201404_8ghw GGACAGAAGCAGTTTGAGCAGGTGTCTATATG(+ACTCAC)GTACCCTGCATCCGGGGCCCTTTGGGAACTG NGG 10 8 17 8 24 0 100 5 10
    240604201402_26w6 CCGGCCAGTTCCCAAAGGGCCCCGGATGCAGG(+ACTCAC)GTACCATATAGACACCTGCTCAAACTGCTTC NGG 10 8 17 8 24 0 100 5 10