Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201505_stpy AGCTTCAGGGTCCTCCTCGCTGTCCACACATG(+ACTCAC)GATCCCAGCGGAATCTCCGCTGACACGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604201503_joud TTTGCCTTCCGTGTCAGCGGAGATTCCGCTGG(+ACTCAC)GATCCATGTGTGGACAGCGAGGAGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240604201501_15pz TAGGCCCCTCCCAGCTGTGAGGGTCCAGGGAG(+ACTCAC)GATCCTGTGGCCGAGTCTTCAGTGGCCGAGT NGG 10 8 17 8 24 0 100 5 10
    240604201459_9220 GCTTTACTCGGCCACTGAAGACTCGGCCACAG(+ACTCAC)GATCCTCCCTGGACCCTCACAGCTGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240604201458_lj8l TGTCAGGAAAAACACATGCCCATGAAGGAATG(+ACTCAC)GTACCCTCTAGTACCACCAAGCTCCACATGA NGG 10 8 17 8 24 0 100 5 10
    240604201456_yrgf ATGGCTCATGTGGAGCTTGGTGGTACTAGAGG(+ACTCAC)GTACCATTCCTTCATGGGCATGTGTTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240604201453_1xc7 ACTGAAATGGGAATGTTCCCAAGGGGCCTGGG(+ACTCAC)GATCCCCTGTGAGGAGAAGTCCTTGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604201451_yqlt AGCTTCAGGCTCAAGGACTTCTCCTCACAGGG(+ACTCAC)GATCCCCAGGCCCCTTGGGAACATTCCCATT NGG 10 8 17 8 24 0 100 5 10
    240604201450_oo22 GGTGGATATCCTGTAACATAAATGCTCGTTTG(+ACTCAC)GATCCAAAAGGAAAACCATTAAAAATAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604201448_gs42 TCTTCCTTTTATTTTTAATGGTTTTCCTTTTG(+ACTCAC)GATCCAAACGAGCATTTATGTTACAGGATAT NGG 10 8 17 8 24 0 100 5 10