Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201538_n7vx GATAGGGAGGGCATGCCTCGCTTTGGATGAGG(+ACTCAC)GATCCATTTAAACACTACCGTTTGGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240604201537_7x6k AGCCCTCAATTCCAAACGGTAGTGTTTAAATG(+ACTCAC)GATCCCTCATCCAAAGCGAGGCATGCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240604201536_orwo ATCCTCTGCCCGCAATGCTTTTGAAAGACTTG(+ACTCAC)GATCCTTTTGAAATGGATGCTGAGCTGCACT NGG 10 8 17 8 24 0 100 5 10
    240604201535_ztyv CGCACAGTGCAGCTCAGCATCCATTTCAAAAG(+ACTCAC)GATCCAAGTCTTTCAAAAGCATTGCGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240604201533_rx1r AGCCAGTGGAACGGAAGGTCCAAGTACCAAAG(+ACTCAC)GATCCTCAGAACACTCCCTACAGTCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604201531_acbw CGGTGCCAGGAGACTGTAGGGAGTGTTCTGAG(+ACTCAC)GATCCTTTGGTACTTGGACCTTCCGTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240604201530_s89k TTAAAGCAACCAAAGACAACTTGGGAACTAAG(+ACTCAC)GATCCTGGAGCACTACAGCCCCTGCAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240604201528_z79k CAGGGGACACTGCAGGGGCTGTAGTGCTCCAG(+ACTCAC)GATCCTTAGTTCCCAAGTTGTCTTTGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240604201527_9w0q GCCTATGTGTGCGAGGACTGTGCATCAGAAAG(+ACTCAC)GTACCACGGACCACAGAGTTCAAACCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240604201525_eptd TTGGGCAGAAGGTTTGAACTCTGTGGTCCGTG(+ACTCAC)GTACCTTTCTGATGCACAGTCCTCGCACACA NGG 10 8 17 8 24 0 100 5 10