Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201557_w84n AAGAGGTCACACGGTTTGGAAACTTGGGGTAG(+ACTCAC)GTACCAGAGCATACAAGTTTAAATCAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240604201554_6086 TGCAATGCCCTGATTTAAACTTGTATGCTCTG(+ACTCAC)GTACCTACCCCAAGTTTCCAAACCGTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240604201552_3f06 CTTACTTAGGTATCAGTAACCTTCAGCTGAGG(+ACTCAC)GATCCACTGGGTAGTGGATTGTGCTATCTGT NGG 10 8 17 8 24 0 100 5 10
    240604201550_4hps TGGATACAGATAGCACAATCCACTACCCAGTG(+ACTCAC)GATCCCTCAGCTGAAGGTTACTGATACCTAA NGG 10 8 17 8 24 0 100 5 10
    240604201548_yvkz GCCCCTGATCCAAGTCTCCAAAAGAGGCCAGG(+ACTCAC)GATCCTTCGAGCAAAGAGATAAATCTTATTC NGG 10 8 17 8 24 0 100 5 10
    240604201546_l943 CACTTGAATAAGATTTATCTCTTTGCTCGAAG(+ACTCAC)GATCCCTGGCCTCTTTTGGAGACTTGGATCA NGG 10 8 17 8 24 0 100 5 10
    240604201545_ezcl TGAGCCCAGCATGGGCGATGGTCATCCTCATG(+ACTCAC)GATCCAGGATAGATGGTCACTCTCAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240604201543_7nx0 CCCAGCCATGCTGAGAGTGACCATCTATCCTG(+ACTCAC)GATCCATGAGGATGACCATCGCCCATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604201541_b979 GGAGATGTTTTTATAAAGCTGATGGCTCCTAG(+ACTCAC)GATCCCTGTGTTTGTCTACAGCTCAACACAG NGG 10 8 17 8 24 0 100 5 10
    240604201540_skmp TACTACTGTGTTGAGCTGTAGACAAACACAGG(+ACTCAC)GATCCTAGGAGCCATCAGCTTTATAAAAACA NGG 10 8 17 8 24 0 100 5 10