Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201523_qcc7 GCACTGATAGGCACTTGCAAAAAAGGCTGGAG(+ACTCAC)GATCCGCAAAGGCCTTGCTGAAGGAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604201522_3bsl TGTTTCCTGGCTCCTTCAGCAAGGCCTTTGCG(+ACTCAC)GATCCTCCAGCCTTTTTTGCAAGTGCCTATC NGG 10 8 17 8 24 0 100 5 10
    240604201521_4k1s GTGTCTCCAGCAGGGCCACAATTCAAAATCTG(+ACTCAC)GATCCCAGGTGTCAGAGGGGACGGGGAATAC NGG 10 8 17 8 24 0 100 5 10
    240604201518_5arz AGTGTGTATTCCCCGTCCCCTCTGACACCTGG(+ACTCAC)GATCCAGATTTTGAATTGTGGCCCTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604201517_x6zd TTAAAAGAGTAGCTTCCTCTGCTGTACTAGTG(+ACTCAC)GATCCTGTTTTTGTTGCTTCCAGGGACCAGC NGG 10 8 17 8 24 0 100 5 10
    240604201515_jwt2 AAAAAGCTGGTCCCTGGAAGCAACAAAAACAG(+ACTCAC)GATCCACTAGTACAGCAGAGGAAGCTACTCT NGG 10 8 17 8 24 0 100 5 10
    240604201513_gta0 TTTACATGAAGTGATGAGACCAAAGGCCCAAG(+ACTCAC)GATCCCCGTCCCCACCCCCCACCCCCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240604201510_07yx GAAAACAAAAGGGGGTGGGGGGTGGGGACGGG(+ACTCAC)GATCCTTGGGCCTTTGGTCTCATCACTTCAT NGG 10 8 17 8 24 0 100 5 10
    240604201508_jesc CAGAGCCAGTTTGGTCTTTCTCCCCCTGCCAG(+ACTCAC)GATCCCAGGCCCTAATCAGTATTATTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240604201506_41ox TTATGAAAGAAATAATACTGATTAGGGCCTGG(+ACTCAC)GATCCTGGCAGGGGGAGAAAGACCAAACTGG NGG 10 8 17 8 24 0 100 5 10