Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201343_xu63 TCTACTTCAGAGGCGATGAGGATAACCTGCAG(+ACTCAC)GATCCTAAGCATGTGCAACAGGCCATGAACC NGG 10 8 17 8 24 0 100 5 10
    240604201342_31zn CCAAAGGTTCATGGCCTGTTGCACATGCTTAG(+ACTCAC)GATCCTGCAGGTTATCCTCATCGCCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240604201341_y30b GCCCCTCATCTCTATCGTTGCTTTTGTTCAAG(+ACTCAC)GATCCAGTCAAGGCTAACATGTTCCGCGGCC NGG 10 8 17 8 24 0 100 5 10
    240604201340_50wk GATGTGGCCGCGGAACATGTTAGCCTTGACTG(+ACTCAC)GATCCTTGAACAAAAGCAACGATAGAGATGA NGG 10 8 17 8 24 0 100 5 10
    240604201339_1gyq GATGCCCCCCCCCCAACCCAGTGTATTCTCTG(+ACTCAC)GATCCCTAAGTGCCTTAGATCCTGAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240604201337_ltzp AGCTCTGTGGCTCAGGATCTAAGGCACTTAGG(+ACTCAC)GATCCAGAGAATACACTGGGTTGGGGGGGGG NGG 10 8 17 8 24 0 100 5 10
    240604201335_9r2h GGGTCCAGGCTCTTCAGGCTCAGTAGGGTCAG(+ACTCAC)GTACCAGCTATACTCTGGTTGTTAAAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240604201334_zk6v ACCCCAAGCCTTTAACAACCAGAGTATAGCTG(+ACTCAC)GTACCTGACCCTACTGAGCCTGAAGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604201333_e4az ATAACTGGCTGGGGGGTTAGGCAAAGGACCTG(+ACTCAC)GTACCCTTACTACTCAAAGCCTACCTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604201331_j7o8 ACTGCCCACTAGGTAGGCTTTGAGTAGTAAGG(+ACTCAC)GTACCAGGTCCTTTGCCTAACCCCCCAGCCA NGG 10 8 17 8 24 0 100 5 10