Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200230_47q8 GTTGGGCAACACTTAGATGGAAAGTTTGTGGG(+ACTCAC)GTACCTGTCCAATCATTTTCCTTCCTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240604200228_h789 CCCTAGCCTAGGGCTTCGACATTGCATGCTGG(+ACTCAC)GATCCAGCTCCTACCCTTCCTGCACTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240604200227_0no0 TGGGGAAGGGAGTGCAGGAAGGGTAGGAGCTG(+ACTCAC)GATCCCAGCATGCAATGTCGAAGCCCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240604200225_vvyu CTAACCACTGCCCTCCTGGAACTGCTGTGCTG(+ACTCAC)GATCCAAAGGCCTCCTCACCAGGAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240604200223_vck0 GGGCCAGAGCCTTCCTGGTGAGGAGGCCTTTG(+ACTCAC)GATCCAGCACAGCAGTTCCAGGAGGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240604200221_rl0x ATAGGTGCTGGAGAAGCGGGGACCGCAGTGGG(+ACTCAC)GTACCATCAGGCTCGGGGTAAGGTAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604200220_zae6 TTACCCTTTTCTACCTTACCCCGAGCCTGATG(+ACTCAC)GTACCCCACTGCGGTCCCCGCTTCTCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604200219_3vas CTCCTCTACACAGCAGCAACTAAAATGATCAG(+ACTCAC)GATCCTTCACTGGAGCCCTAGAGACTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604200218_wqp9 ATCTTCTGGGAGTCTCTAGGGCTCCAGTGAAG(+ACTCAC)GATCCTGATCATTTTAGTTGCTGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240604200216_akfp CATACCTAGGAGCAAGCACTGATCGCTGAGCG(+ACTCAC)GTACCAAGAGGAGCCTCTCCTTTTACTGGAT NGG 10 8 17 8 24 0 100 5 10