Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200334_e0m6 TGACCTCAGCAAATCCTCCTTTTAACTCATGG(+ACTCAC)GTACCCAGACTCGAACTATTTCACAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240604200332_qn9c TGGTTGTGAATTGTGAAATAGTTCGAGTCTGG(+ACTCAC)GTACCCATGAGTTAAAAGGAGGATTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604200330_ycf8 AAACTTGGCTGACTTAGAACTGGAAGTTGCTG(+ACTCAC)GTACCCAGGCTGAAATCTCTTGCGCTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240604200328_16hl TCAAGAAGCCAGCGCAAGAGATTTCAGCCTGG(+ACTCAC)GTACCAGCAACTTCCAGTTCTAAGTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240604200326_qije TGTGGATAACATATTATGTATATATGTAATGG(+ACTCAC)GTACCAGGCCAGGTTTATATTTTTTTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240604200324_szu9 ACGTCTAAAGAAAAAAATATAAACCTGGCCTG(+ACTCAC)GTACCCATTACATATATACATAATATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240604200322_52d7 GCTCACAACCATGCATAACTCAAGTTTTAAGG(+ACTCAC)GATCCAGTGCTGACTTCTGGCCTCTGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240604200321_mh2m GCAGTACCCTCAGAGGCCAGAAGTCAGCACTG(+ACTCAC)GATCCCTTAAAACTTGAGTTATGCATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240604200320_o055 TTGGGGGAGATGCAGCACACACCCCAGATAGG(+ACTCAC)GATCCTATGACTGACCAACAAAAGTACAGGT NGG 10 8 17 8 24 0 100 5 10
    240604200319_7071 GTGGTACCTGTACTTTTGTTGGTCAGTCATAG(+ACTCAC)GATCCCTATCTGGGGTGTGTGCTGCATCTCC NGG 10 8 17 8 24 0 100 5 10