Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200439_2p6f CCACAGACCCATTGGGGCCAGGTCTAGCTCAG(+ACTCAC)GTACCTGAGCAAATGTTCCTTTAGAGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240604200438_mb0v CCCTGGTGGACTCTAAAGGAACATTTGCTCAG(+ACTCAC)GTACCTGAGCTAGACCTGGCCCCAATGGGTC NGG 10 8 17 8 24 0 100 5 10
    240604200436_op7f ACTTGTTAAGAGGCAAGGCCACCTTTCACCAG(+ACTCAC)GTACCCTGGCCAGGCACCTGGGAAGACTCTT NGG 10 8 17 8 24 0 100 5 10
    240604200434_uzd2 GCCAGAAGAGTCTTCCCAGGTGCCTGGCCAGG(+ACTCAC)GTACCTGGTGAAAGGTGGCCTTGCCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240604200432_itqu TCCTGGGCAGCCTGGAGGGCTGGCATGCCTGG(+ACTCAC)GATCCTCGGCCACCCAGGATCTTGGAGACAA NGG 10 8 17 8 24 0 100 5 10
    240604200430_soap GACACTTGTCTCCAAGATCCTGGGTGGCCGAG(+ACTCAC)GATCCCAGGCATGCCAGCCCTCCAGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240604200427_7c89 GCCAGGACAGTCTTCTTCAGCTGGGCAGGCGG(+ACTCAC)GATCCTGGGAAAGCGAAGTGGCAGAAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604200425_kn0v CAATGCCAGCTTCTGCCACTTCGCTTTCCCAG(+ACTCAC)GATCCCGCCTGCCCAGCTGAAGAAGACTGTC NGG 10 8 17 8 24 0 100 5 10
    240604200424_6ok6 TCACACAATAAGATTAAGTTTTAGAAACTAAG(+ACTCAC)GATCCCACCTTTTTTTCTTGTTTAAAATAAT NGG 10 8 17 8 24 0 100 5 10
    240604200422_xmz8 AATAAATTATTTTAAACAAGAAAAAAAGGTGG(+ACTCAC)GATCCTTAGTTTCTAAAACTTAATCTTATTG NGG 10 8 17 8 24 0 100 5 10