Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201017_k2na CCTTTTAGCTGCCATCTGCCAAGCACACCTGG(+ACTCAC)GATCCACCAGTTCTGGCAATGCTGTGACAGT NGG 10 8 17 8 24 0 100 5 10
    240604201015_car8 CAGTCACTGTCACAGCATTGCCAGAACTGGTG(+ACTCAC)GATCCCAGGTGTGCTTGGCAGATGGCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240604201014_x4of AAAAAAAAAAAATTCCCGGGAGCAACTGCCTG(+ACTCAC)GATCCTTTCCCTACAGTCGCTCGATCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240604201012_c1ry CTGTCTGGGAGATCGAGCGACTGTAGGGAAAG(+ACTCAC)GATCCAGGCAGTTGCTCCCGGGAATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604201011_rx67 CCTTGGTTCTGTTGCTTACTGGCCGAGAAACG(+ACTCAC)GTACCAATCTTTCAGCTCTGTGCTTGTCGGC NGG 10 8 17 8 24 0 100 5 10
    240604201010_w4uv AAGAAGCCGACAAGCACAGAGCTGAAAGATTG(+ACTCAC)GTACCGTTTCTCGGCCAGTAAGCAACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240604201008_0q6o TCGTCAGCAGCCTGTCTCATTGCTTCAGCTGG(+ACTCAC)GTACCAGTCCTTCTGTTCCCCTGGGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240604201007_r70o GAGTTCAGTTTCCCAGGGGAACAGAAGGACTG(+ACTCAC)GTACCCAGCTGAAGCAATGAGACAGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240604201005_cik1 TGCAATCCCAGGGCTGGGAGATGGAGCCAGAG(+ACTCAC)GATCCCTGGGACTTGCTGGCCAGCCAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240604201002_vhwd TTAACTAGCCTGGCTGGCCAGCAAGTCCCAGG(+ACTCAC)GATCCTCTGGCTCCATCTCCCAGCCCTGGGA NGG 10 8 17 8 24 0 100 5 10