Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200607_s652 TCTCTAGCACCTGGCTCCTGTGGCTCTCTATG(+ACTCAC)GTACCTGGAATCCCCAAGGTGTTTAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240604200605_uv6a GAGAAAGGAGTTAAACACCTTGGGGATTCCAG(+ACTCAC)GTACCATAGAGAGCCACAGGAGCCAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240604200603_wb4d ATCTTACAGTCAGCAATCTGGGGGTGGGGAGG(+ACTCAC)GATCCCTCTCTAGCACCTGGCTCCTGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240604200601_4shb TAGAGAGCCACAGGAGCCAGGTGCTAGAGAGG(+ACTCAC)GATCCCTCCCCACCCCCAGATTGCTGACTGT NGG 10 8 17 8 24 0 100 5 10
    240604200559_vor6 GGCAAAGACTGAGCTCAGGCACCAGCCTCCCG(+ACTCAC)GTACCACGCTGGAGGTGGATCCCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240604200557_igbm TGCCTTCTACTCAGGGATCCACCTCCAGCGTG(+ACTCAC)GTACCGGGAGGCTGGTGCCTGAGCTCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240604200556_2xcf GGCACCAGCCTCCCGGTACCACGCTGGAGGTG(+ACTCAC)GATCCCTGAGTAGAAGGCAGGGTAAGCTTCG NGG 10 8 17 8 24 0 100 5 10
    240604200554_us1l GAAGTCGAAGCTTACCCTGCCTTCTACTCAGG(+ACTCAC)GATCCACCTCCAGCGTGGTACCGGGAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604200552_ucdu CTGTGCCAGAGCCGCTAGCAGCTGCTCACAGG(+ACTCAC)GTACCTTTGTGGGTCTGGTGTTTTGAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240604200551_s6ab GGGTGATTTCTCAAAACACCAGACCCACAAAG(+ACTCAC)GTACCCTGTGAGCAGCTGCTAGCGGCTCTGG NGG 10 8 17 8 24 0 100 5 10