Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200716_aql3 TGACAGGCTCTGGAGCTGCCTCCTAGGCTGGG(+ACTCAC)GTACCCCTAGGCCCTGGCAACCCACGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240604200714_bu16 TGTAAAACAACGTGGGTTGCCAGGGCCTAGGG(+ACTCAC)GTACCCCAGCCTAGGAGGCAGCTCCAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604200712_y32i TGGTCCTATTCTAGAGAAGAAAGCCTTGCTGG(+ACTCAC)GTACCAATTATGGACTATGGGAGGTAGGATG NGG 10 8 17 8 24 0 100 5 10
    240604200711_ztj9 AGGAGCATCCTACCTCCCATAGTCCATAATTG(+ACTCAC)GTACCCAGCAAGGCTTTCTTCTCTAGAATAG NGG 10 8 17 8 24 0 100 5 10
    240604200709_u7dm GGCTACATGCACGGGCTTCTCTGAGATTCCAG(+ACTCAC)GTACCAGTTCCTTCCGGGTTATGTAATATTA NGG 10 8 17 8 24 0 100 5 10
    240604200708_6d7i GTCTCTAATATTACATAACCCGGAAGGAACTG(+ACTCAC)GTACCTGGAATCTCAGAGAAGCCCGTGCATG NGG 10 8 17 8 24 0 100 5 10
    240604200706_e95c CACACCGGAAGTGAGAGGTGTCTCAGTGGTAG(+ACTCAC)GTACCAATCTCCAACCAAGACCCAGGCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604200705_9ius TTCAGGGAAGCCTGGGTCTTGGTTGGAGATTG(+ACTCAC)GTACCTACCACTGAGACACCTCTCACTTCCG NGG 10 8 17 8 24 0 100 5 10
    240604200702_pdhw GCAGGCAGCAAGGAGACCTGAGGTGGCCCTGG(+ACTCAC)GTACCAGGACCCCAGAGTAGAGGTGAGGATG NGG 10 8 17 8 24 0 100 5 10
    240604200700_e8ye CTGTGCATCCTCACCTCTACTCTGGGGTCCTG(+ACTCAC)GTACCCAGGGCCACCTCAGGTCTCCTTGCTG NGG 10 8 17 8 24 0 100 5 10