Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200733_p51f ACTCAGAAAAGAACCATAGGCACACACGTGTG(+ACTCAC)GATCCTATATCTTGATATTATATAGATACAC NGG 10 8 17 8 24 0 100 5 10
    240604200732_97of GGATGGTGTATCTATATAATATCAAGATATAG(+ACTCAC)GATCCACACGTGTGTGCCTATGGTTCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240604200730_b1d5 GGCTATTCCTTGTCCCTACACTGCTCTCTTGG(+ACTCAC)GTACCTCTGGGGCACCCTGGCAGGAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240604200728_mt0w AAAGTTCTCATTCCTGCCAGGGTGCCCCAGAG(+ACTCAC)GTACCCAAGAGAGCAGTGTAGGGACAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604200726_3wyw GTGGTACAGGGTCCTGGTCTGCTGTGTGTGGG(+ACTCAC)GATCCTGGCTGCAGAGCTTCTTCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240604200724_hz5y TACCTTCTACTCAGAAGAAGCTCTGCAGCCAG(+ACTCAC)GATCCCCACACACAGCAGACCAGGACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604200723_ubhc CAGCAGTTTTAAAGTCTAACACATTTTAAACG(+ACTCAC)GATCCAAAGTCTTCTTTCCTCATCCGTCACT NGG 10 8 17 8 24 0 100 5 10
    240604200722_hpnb TGCTAAGTGACGGATGAGGAAAGAAGACTTTG(+ACTCAC)GATCCGTTTAAAATGTGTTAGACTTTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240604200720_ztq1 GGCACTGGAGGAAACTATTTCTCCCTCTCAAG(+ACTCAC)GATCCGGGCGTGCTCTGCACCCGGCCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240604200718_chw5 AGGCCACCAAGGCCGGGTGCAGAGCACGCCCG(+ACTCAC)GATCCTTGAGAGGGAGAAATAGTTTCCTCCA NGG 10 8 17 8 24 0 100 5 10