Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200804_cpvh CTGCTCTGGCTGACATGGTATATCAGAGGTTG(+ACTCAC)GATCCTCTGAAACTGGATTTATGGGTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240604200803_a5xx GCTCACAACAACCCATAAATCCAGTTTCAGAG(+ACTCAC)GATCCAACCTCTGATATACCATGTCAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604200802_er4k AATCCCAGGGAACCATTCTAGGTCTCTTCTTG(+ACTCAC)GATCCACAGGCCCGATGTCTATGCTCCGGTG NGG 10 8 17 8 24 0 100 5 10
    240604200800_a4f5 GGAAACACCGGAGCATAGACATCGGGCCTGTG(+ACTCAC)GATCCAAGAAGAGACCTAGAATGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604200758_in7d AATCCCAGGGAACCGTTCTAGGTCTCTTCTTG(+ACTCAC)GATCCACAGGCCCGATGTCTATGCCCCGGTG NGG 10 8 17 8 24 0 100 5 10
    240604200756_u5ni GGGAACACCGGGGCATAGACATCGGGCCTGTG(+ACTCAC)GATCCAAGAAGAGACCTAGAACGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604200755_woiw AATCCCAGGGAACCGTTCTAGGTCTCTTCTTG(+ACTCAC)GATCCACAGGCCCGATGTCTATGCCCCGGTG NGG 10 8 17 8 24 0 100 5 10
    240604200753_6bl0 GGGAACACCGGGGCATAGACATCGGGCCTGTG(+ACTCAC)GATCCAAGAAGAGACCTAGAACGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604200751_az4u TAGATGGGCAGCAATGGGCAGTTGCAACCAAG(+ACTCAC)GTACCCAGCAGTGTCTTCTACATCACCTATA NGG 10 8 17 8 24 0 100 5 10
    240604200750_spb5 CTGGATATAGGTGATGTAGAAGACACTGCTGG(+ACTCAC)GTACCTTGGTTGCAACTGCCCATTGCTGCCC NGG 10 8 17 8 24 0 100 5 10