Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200851_ictq CAACTTAGGGGGTGAGCAGGTGTTGGTGATGG(+ACTCAC)GATCCCTGTGATGAGATGGGGGTGACACTGC NGG 10 8 17 8 24 0 100 5 10
    240604200849_wh9g AATGTGCAGTGTCACCCCCATCTCATCACAGG(+ACTCAC)GATCCCATCACCAACACCTGCTCACCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240604200847_5f9u TCGAATATTTAGAAGTGTAAAACATAAACCAG(+ACTCAC)GATCCTGCCCCCCACATGTCCAGGACGATGA NGG 10 8 17 8 24 0 100 5 10
    240604200845_i047 AACACTCATCGTCCTGGACATGTGGGGGGCAG(+ACTCAC)GATCCTGGTTTATGTTTTACACTTCTAAATA NGG 10 8 17 8 24 0 100 5 10
    240604200843_4ur4 TAAATTTCACCCTCTGCATTACTCACAGAGTG(+ACTCAC)GATCCATTGGAGTTTCAGCTGTATGCTAGTT NGG 10 8 17 8 24 0 100 5 10
    240604200841_rj9z AGCTGAACTAGCATACAGCTGAAACTCCAATG(+ACTCAC)GATCCACTCTGTGAGTAATGCAGAGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240604200839_h3n7 GCCAACATTCCTAAGACTGAACCAACATCCAG(+ACTCAC)GTACCCATTTTCTCAGGTTTCCCAGAGATAT NGG 10 8 17 8 24 0 100 5 10
    240604200837_cmub GCCACATATCTCTGGGAAACCTGAGAAAATGG(+ACTCAC)GTACCTGGATGTTGGTTCAGTCTTAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240604200836_97k6 AGAGAAGAACTCTTATAGACTTGGGGAGAGAG(+ACTCAC)GTACCAACCTTGACATTGACTGTACTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240604200835_xtvz AATACTCACCAGTACAGTCAATGTCAAGGTTG(+ACTCAC)GTACCTCTCTCCCCAAGTCTATAAGAGTTCT NGG 10 8 17 8 24 0 100 5 10