Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195646_onaw GGAGGTAGGAACTGATGTAGGGGCCATGGAGG(+ACTCAC)GATCCTGCTTACTGGCTTGCTTCTCATGACT NGG 10 8 17 8 24 0 100 5 10
    240604195644_8tgg CAAAAGTCAATCTCCAACCGTCCAATGTCTGG(+ACTCAC)GATCCAACTTACGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240604195643_nq08 GCCCTTGGAGGAGCCCAGAAGATCGTAAGTTG(+ACTCAC)GATCCCAGACATTGGACGGTTGGAGATTGAC NGG 10 8 17 8 24 0 100 5 10
    240604195642_knbi AGGCAGCCTTGCTTACCGTATGTGAGGTCAGG(+ACTCAC)GTACCGGGTGCTGGGGATTGCTTGTGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240604195639_pieu GTGAGTGGGCCACAAGCAATCCCCAGCACCCG(+ACTCAC)GTACCCTGACCTCACATACGGTAAGCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240604195637_xmpa ATAGCAGGTCCTTAAGTGGGAATGAGCTCAGG(+ACTCAC)GATCCTCAGCCAGCCATGCAGGCAGGGCGCA NGG 10 8 17 8 24 0 100 5 10
    240604195636_umbw GTCGTTGCGCCCTGCCTGCATGGCTGGCTGAG(+ACTCAC)GATCCCTGAGCTCATTCCCACTTAAGGACCT NGG 10 8 17 8 24 0 100 5 10
    240604195635_qj6j ACAGCATAGCAGAAGTTTTTAATAGGTGACTG(+ACTCAC)GTACCACAGTAAGAAGGAATCATTTCGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604195633_e03j ACGGGCAGGCGAAATGATTCCTTCTTACTGTG(+ACTCAC)GTACCAGTCACCTATTAAAAACTTCTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240604195632_5d5i TCAGCTGTGCTCCAGATGATACAACGCAGTTG(+ACTCAC)GTACCTGCAGCCACTGTGCTCTGAGGCTAGG NGG 10 8 17 8 24 0 100 5 10