Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195539_4dlu CATGCCAGTCTCCAGGACCCTTTGGTGGTGAG(+ACTCAC)GATCCTGCCAGGGGTTCTCTGCGCTCCACAC NGG 10 8 17 8 24 0 100 5 10
    240604195537_3r85 TACTCTACTCCATACCCAGCAGCCCTAGAAGG(+ACTCAC)GATCCTGGCCTGCTTTACAGAGAAATGAATG NGG 10 8 17 8 24 0 100 5 10
    240604195535_k4tv AACTTCATTCATTTCTCTGTAAAGCAGGCCAG(+ACTCAC)GATCCCTTCTAGGGCTGCTGGGTATGGAGTA NGG 10 8 17 8 24 0 100 5 10
    240604195533_hhx0 GTTAGAGGTCTCTCTGCCCAGCGCCACACAGG(+ACTCAC)GATCCCGGCACAGAGCTCTGAGCGTTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604195531_om31 TGTCCCCAGGAACGCTCAGAGCTCTGTGCCGG(+ACTCAC)GATCCCTGTGTGGCGCTGGGCAGAGAGACCT NGG 10 8 17 8 24 0 100 5 10
    240604195530_sf4p CTCTCCTTCTCCAGTGCTTATAGACATGTATG(+ACTCAC)GATCCTGTTCTGCTGCTGGGAAAGCCACTGG NGG 10 8 17 8 24 0 100 5 10
    240604195529_vdhh GTCCCCCAGTGGCTTTCCCAGCAGCAGAACAG(+ACTCAC)GATCCATACATGTCTATAAGCACTGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604195527_6k13 CCCATCAGTCTTTAGCCCCAGGCTCAGGCAGG(+ACTCAC)GATCCCTTCATCTGATCCTCTACCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240604195525_p4vr GTGGGGAGGAGGGGTAGAGGATCAGATGAAGG(+ACTCAC)GATCCCTGCCTGAGCCTGGGGCTAAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240604195523_dkog GCTCTCGCAGAAGATGGAGGTTCAATCTCCAG(+ACTCAC)GATCCAGATGATGGCTCCCAACTGTCTGTAA NGG 10 8 17 8 24 0 100 5 10