Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195448_huz2 AGGGCTCTGGTGGCATGCTCTCAGCGTCAGTG(+ACTCAC)GTACCCACCAGCTAACTATCAGCAAAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240604195446_oq9i TTCCACAGTGGCATGGCCACCCAGCCTCGGGG(+ACTCAC)GATCCTGGGCTCGCTGCACAGCACCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240604195444_krs5 GAAGAGATCTGGGTGCTGTGCAGCGAGCCCAG(+ACTCAC)GATCCCCCGAGGCTGGGTGGCCATGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240604195442_dyh4 AGCCTCCACACGAGTGTTCTAGTACATGTTAG(+ACTCAC)GATCCTCTCCTGTACCAGTGTCCAGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240604195441_kwmo GTCTGTTACACCTGGACACTGGTACAGGAGAG(+ACTCAC)GATCCTAACATGTACTAGAACACTCGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240604195440_y869 TTCCACTCAAATCAGTTGAGAGCCCAAATCAG(+ACTCAC)GATCCACATATCATTCATTGTCATTAGATCT NGG 10 8 17 8 24 0 100 5 10
    240604195438_v4a7 TATTGAGATCTAATGACAATGAATGATATGTG(+ACTCAC)GATCCTGATTTGGGCTCTCAACTGATTTGAG NGG 10 8 17 8 24 0 100 5 10
    240604195437_6n38 GCTGTTAACAGGAGGTCCAAACCTCTTGGAAG(+ACTCAC)GTACCAAGCAGCTAATAAAGGCTGGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604195435_46gk GATGAAGAAGACCAGCCTTTATTAGCTGCTTG(+ACTCAC)GTACCTTCCAAGAGGTTTGGACCTCCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240604195434_qipd AGTCTCCCTAGCGCCACTGTGCTGAGAACAGG(+ACTCAC)GATCCTCTGGATGAGGAAGCCCTGGCCCCAG NGG 10 8 17 8 24 0 100 5 10