Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195401_hd3u TTTCTTGGCATTTTCCATTCATAACTGAAGAG(+ACTCAC)GATCCACATGTATTGCAAGCCAGGAGTAGGA NGG 10 8 17 8 24 0 100 5 10
    240604195359_b8oy ATAACATTAACCACATTCTGTTATATAGACTG(+ACTCAC)GATCCTTAAAATACACTAATTTATAAATACT NGG 10 8 17 8 24 0 100 5 10
    240604195358_1mrz TTTTCAGTATTTATAAATTAGTGTATTTTAAG(+ACTCAC)GATCCAGTCTATATAACAGAATGTGGTTAAT NGG 10 8 17 8 24 0 100 5 10
    240604195357_u3bh GTTGGTGTTTACATTGCTCTGCTCTGTGAAAG(+ACTCAC)GATCCTGCAGCAGAGGCTTCCTACCTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604195356_sjxk AACATTTTTCAGGTAGGAAGCCTCTGCTGCAG(+ACTCAC)GATCCTTTCACAGAGCAGAGCAATGTAAACA NGG 10 8 17 8 24 0 100 5 10
    240604195354_mhdt GGCTGATGCCTGTCAAGATGACAACAGGAGGG(+ACTCAC)GATCCACCAAGGTAGAAAACATCCCTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604195352_8ity TCTTGTCAGCAGGGATGTTTTCTACCTTGGTG(+ACTCAC)GATCCCCTCCTGTTGTCATCTTGACAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604195351_nsow AGGTGAACGTTCCACTGTAAAAGGCACTACTG(+ACTCAC)GATCCGAGAGGGTTTATTTTAAGCATGTGCA NGG 10 8 17 8 24 0 100 5 10
    240604195350_7wwd TCTGCTGCACATGCTTAAAATAAACCCTCTCG(+ACTCAC)GATCCAGTAGTGCCTTTTACAGTGGAACGTT NGG 10 8 17 8 24 0 100 5 10
    240604195348_xisk GAACATGGGGGGTGAAATGGTGCTGAGGGGAG(+ACTCAC)GTACCGACTCAGTGTCCACGCTCACTGTCAT NGG 10 8 17 8 24 0 100 5 10