Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195330_nh1x CTGTGATTTGCAAGTATTGTAAATGGATGCAG(+ACTCAC)GTACCTGGTGTTGCTTTTAACTTTTACTGTC NGG 10 8 17 8 24 0 100 5 10
    240604195327_a7d0 CAATTTTACAAATAAATACTTTCTTTCCTTGG(+ACTCAC)GTACCTAGAATAATTAATCCAGCATACAACT NGG 10 8 17 8 24 0 100 5 10
    240604195325_1gry TTCAAAGTTGTATGCTGGATTAATTATTCTAG(+ACTCAC)GTACCCAAGGAAAGAAAGTATTTATTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240604195323_076p TAAATGGGCTTCTCAGATAGTACCCTCCACAG(+ACTCAC)GTACCCCCTTAATCAATACCATGCAAATTGT NGG 10 8 17 8 24 0 100 5 10
    240604195321_d5ah TGAGCACAATTTGCATGGTATTGATTAAGGGG(+ACTCAC)GTACCTGTGGAGGGTACTATCTGAGAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604195320_ollj TAAGCCTTCCTTCATTCTGAATACTTCACTTG(+ACTCAC)GTACCATGCTAGGACTGAGAGTCATATTTCA NGG 10 8 17 8 24 0 100 5 10
    240604195319_rqmo CTTTGTGAAATATGACTCTCAGTCCTAGCATG(+ACTCAC)GTACCAAGTGAAGTATTCAGAATGAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604195317_idrb ACTGCGATTCTCCAATGGGTTACGTGCCAATG(+ACTCAC)GTACCTACAATGTATTGTTATAAGACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240604195315_5yzk TCCATTCTAGGTCTTATAACAATACATTGTAG(+ACTCAC)GTACCATTGGCACGTAACCCATTGGAGAATC NGG 10 8 17 8 24 0 100 5 10
    240604195313_7w48 CTGTGGTTAGTCACAGATGCAAGAGATCCAAG(+ACTCAC)GATCCGAGGCTTTGTGAGCACAGTAATTAAG NGG 10 8 17 8 24 0 100 5 10