Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195416_ld6y AAACATGTGGACACGCCATTTCTGAGACACAG(+ACTCAC)GTACCTCCTTCACAGCTACTGCCTGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240604195415_mbb1 ACCAAGGGGGGAAATCCCAGCAGATAATTTAG(+ACTCAC)GATCCCCTTCTAAACACATGAGGGCCTGTAT NGG 10 8 17 8 24 0 100 5 10
    240604195413_mtte TGCACATACAGGCCCTCATGTGTTTAGAAGGG(+ACTCAC)GATCCTAAATTATCTGCTGGGATTTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240604195412_fe1t AATGTTTAGAGAACAATTAGGTCAATTTCAGG(+ACTCAC)GATCCACTTGGTGTATGTGTGGACCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240604195410_oyo9 ACCTATCTGTAGGTCCACACATACACCAAGTG(+ACTCAC)GATCCCTGAAATTGACCTAATTGTTCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240604195409_3gev ATGCCTACTTGATTTCTATTAAAAAGAAAAAG(+ACTCAC)GTACCAGGTAATTCTATTTGGGAAACGAATG NGG 10 8 17 8 24 0 100 5 10
    240604195407_izsu TAGTGCATTCGTTTCCCAAATAGAATTACCTG(+ACTCAC)GTACCTTTTTCTTTTTAATAGAAATCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240604195405_h371 GTATTTACGGATAGTTGTAAGCCACTGTGTGG(+ACTCAC)GTACCGGGAATTGAACCCAGGTCCTCTGCTA NGG 10 8 17 8 24 0 100 5 10
    240604195403_oa9o AGCACTAGCAGAGGACCTGGGTTCAATTCCCG(+ACTCAC)GTACCCACACAGTGGCTTACAACTATCCGTA NGG 10 8 17 8 24 0 100 5 10
    240604195402_ng4u TAATGTCCTACTCCTGGCTTGCAATACATGTG(+ACTCAC)GATCCTCTTCAGTTATGAATGGAAAATGCCA NGG 10 8 17 8 24 0 100 5 10