Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195521_gebq TGGAGTTACAGACAGTTGGGAGCCATCATCTG(+ACTCAC)GATCCTGGAGATTGAACCTCCATCTTCTGCG NGG 10 8 17 8 24 0 100 5 10
    240604195520_dqn8 ACACACACACACCACACGCAAAGCCATGGGAG(+ACTCAC)GATCCCAGAGGCCTTGTTGAGAATTGGATTC NGG 10 8 17 8 24 0 100 5 10
    240604195518_mv1j CTCATGAATCCAATTCTCAACAAGGCCTCTGG(+ACTCAC)GATCCTCCCATGGCTTTGCGTGTGGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604195516_52ns ACAAGGACACTGGGAGGTGTGTGAACGCCATG(+ACTCAC)GTACCGGCGGCTGCTTGAGGCGTTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604195513_kcf6 GAGTCACAGGAAAACGCCTCAAGCAGCCGCCG(+ACTCAC)GTACCATGGCGTTCACACACCTCCCAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240604195511_63tl GGTGCTCCCAGGGCACTCACCACAGCCACCAG(+ACTCAC)GATCCTCCAGAGGCAAGCAGCATCTCAGATT NGG 10 8 17 8 24 0 100 5 10
    240604195509_rxx8 CTTGTAATCTGAGATGCTGCTTGCCTCTGGAG(+ACTCAC)GATCCTGGTGGCTGTGGTGAGTGCCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604195508_cqe0 AAATCACGTCTCACAGCAGGCACCACTGTGTG(+ACTCAC)GATCCAAGTCCCCCACCTTACCCTGCACCAG NGG 10 8 17 8 24 0 100 5 10
    240604195507_q3gj CGCCCCTGGTGCAGGGTAAGGTGGGGGACTTG(+ACTCAC)GATCCACACAGTGGTGCCTGCTGTGAGACGT NGG 10 8 17 8 24 0 100 5 10
    240604195505_nb9c ACCACAAATTTATTGTTTATAGCTGAGATCAG(+ACTCAC)GATCCGAGCAATCCCGTTTCAGCTGCCCAGT NGG 10 8 17 8 24 0 100 5 10