Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194546_ralb CTTAGAGCAGTTTCCTTAACTAACAGAAGCAG(+ACTCAC)GATCCTTATGTGATCTTAAACCTTCATTTGA NGG 10 8 17 8 24 0 100 5 10
    240604194545_xqjq TGATGCAGCTGACTGATGAAGGTACTGGTGAG(+ACTCAC)GTACCTCTAGGGCTAGGACCAGATGCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240604194543_bo02 TGAAGTCACTGCATCTGGTCCTAGCCCTAGAG(+ACTCAC)GTACCTCACCAGTACCTTCATCAGTCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604194541_sjcp GTCTTCTTCTCTGCCAGATAACAGAGCCATTG(+ACTCAC)GATCCACTTATTAAAGACTTCTTATCACTTA NGG 10 8 17 8 24 0 100 5 10
    240604194540_20wg AATGTTAAGTGATAAGAAGTCTTTAATAAGTG(+ACTCAC)GATCCAATGGCTCTGTTATCTGGCAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604194539_qjaj CTTTTTCTAGAGTCCTTTCTACACACACTTTG(+ACTCAC)GTACCCTGGGGAGTTTGGATGCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604194536_bp6w CCTCCTTCCTGGTTGCATCCAAACTCCCCAGG(+ACTCAC)GTACCAAAGTGTGTGTAGAAAGGACTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240604194535_6w6i CAAGACCTTCAAGAAGGTGGGCTCCGGGGATG(+ACTCAC)GTACCATTGCTCTTTCCTAGCTAACCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240604194533_g8p6 CCGTATCTAGGGTTAGCTAGGAAAGAGCAATG(+ACTCAC)GTACCATCCCCGGAGCCCACCTTCTTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604194532_jsk7 AAATGGTCCATATAAATTTGTGGCATCAATGG(+ACTCAC)GATCCCAACTTGGCTTCCCTAGTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10