Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194457_j0hl GCTCCAGACTCCAAGATCTTGGACCATTCAGG(+ACTCAC)GATCCAAAGGAGATTCATTAAGACTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240604194455_wzfp AAAGTATGAATGTGTTCTGCACAGCAAACTGG(+ACTCAC)GATCCTTATCAGTACAACCTGAAGACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240604194454_pf3v AGGGACTCTAGTCTTCAGGTTGTACTGATAAG(+ACTCAC)GATCCCAGTTTGCTGTGCAGAACACATTCAT NGG 10 8 17 8 24 0 100 5 10
    240604194452_i782 AGAATTATCAAGGAACACCTAAAAATGACCAG(+ACTCAC)GATCCAAATAGGTTATTTATATTATATTTCT NGG 10 8 17 8 24 0 100 5 10
    240604194451_51yi TATAGAGAAATATAATATAAATAACCTATTTG(+ACTCAC)GATCCTGGTCATTTTTAGGTGTTCCTTGATA NGG 10 8 17 8 24 0 100 5 10
    240604194449_6ybb AGCTGAGTTTTGCATAATGATTTTGTATCTTG(+ACTCAC)GTACCTCACTGAATTCATTTATGATTTATGA NGG 10 8 17 8 24 0 100 5 10
    240604194448_3i61 ATCCATCATAAATCATAAATGAATTCAGTGAG(+ACTCAC)GTACCAAGATACAAAATCATTATGCAAAACT NGG 10 8 17 8 24 0 100 5 10
    240604194446_ej05 TATAATAAAATAATACTAAAAGAGAGCATGTG(+ACTCAC)GATCCATACACCAGATTAATGCGGGGATAGG NGG 10 8 17 8 24 0 100 5 10
    240604194445_yx6y CATACCCTATCCCCGCATTAATCTGGTGTATG(+ACTCAC)GATCCACATGCTCTCTTTTAGTATTATTTTA NGG 10 8 17 8 24 0 100 5 10
    240604194443_mcrk CGACAAACGTGCATTTGATAGCACTATTTTAG(+ACTCAC)GTACCCAGAAGCAGACAATACAGCAGTGAAT NGG 10 8 17 8 24 0 100 5 10