Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194414_a3w0 GAAATTCTCTGACTAGACCCTGTTTCCTAATG(+ACTCAC)GTACCAATGGGCTGAGGACCAAAACTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240604194413_i6f8 CGGACTAGGTGGTTAGTGGATACTATTGTTTG(+ACTCAC)GATCCTGACTGTTACAGGGTTAAAGTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240604194412_pue0 GAGGACCAAAACTTTAACCCTGTAACAGTCAG(+ACTCAC)GATCCAAACAATAGTATCCACTAACCACCTA NGG 10 8 17 8 24 0 100 5 10
    240604194409_jl1b GAGAAAGCCTAGAGTCTCCCCTTACTCCAGGG(+ACTCAC)GTACCAGCTTCAGAAGAAGGTGTGAGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240604194408_7pdp GAAGTGTGAGCTCACACCTTCTTCTGAAGCTG(+ACTCAC)GTACCCCTGGAGTAAGGGGAGACTCTAGGCT NGG 10 8 17 8 24 0 100 5 10
    240604194405_gra5 CCTGGTATACCGGGAAGGTGGTCTAGCCTAGG(+ACTCAC)GTACCAGGGCTGCAGGTGAAACCTGAACTTT NGG 10 8 17 8 24 0 100 5 10
    240604194403_xl3b GGGTAAAAGTTCAGGTTTCACCTGCAGCCCTG(+ACTCAC)GTACCCTAGGCTAGACCACCTTCCCGGTATA NGG 10 8 17 8 24 0 100 5 10
    240604194402_e7a3 CCTGGCCTGTTGTCCTTTACCTACCTAAGCTG(+ACTCAC)GTACCTTTATTACTGTTGCATTGTACTGAAC NGG 10 8 17 8 24 0 100 5 10
    240604194401_ic6r ACTGGGTTCAGTACAATGCAACAGTAATAAAG(+ACTCAC)GTACCAGCTTAGGTAGGTAAAGGACAACAGG NGG 10 8 17 8 24 0 100 5 10
    240604194359_cyd4 GTAACCCCCTCCTTCCAACTTCTTAGGAGAAG(+ACTCAC)GATCCAAGCACGTATCTGTGCACACAAATTC NGG 10 8 17 8 24 0 100 5 10