Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194342_bhnu TTGTGGAAGTAGATGCCCAGGAATCCTTTAAG(+ACTCAC)GTACCTGTTAGATGGCAATTTTGATCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240604194341_cfq0 TTGGGTTGATTTTCCTGAATTGGTTCCTATAG(+ACTCAC)GATCCTCCAATTTTGGATCTTCTACAGATCC NGG 10 8 17 8 24 0 100 5 10
    240604194339_scvy CTGGGGGATCTGTAGAAGATCCAAAATTGGAG(+ACTCAC)GATCCTATAGGAACCAATTCAGGAAAATCAA NGG 10 8 17 8 24 0 100 5 10
    240604194338_l5ba TGAGGCACAGTCTCTGTTCTGGAATTCACAAG(+ACTCAC)GTACCTGTAATAAACATCCAAACAAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240604194337_nix3 ATTTGTGACTCTTGTTTGGATGTTTATTACAG(+ACTCAC)GTACCTTGTGAATTCCAGAACAGAGACTGTG NGG 10 8 17 8 24 0 100 5 10
    240604194335_wq4j TCTAATGTAATAATGTTTTTTAAAGCACTCTG(+ACTCAC)GTACCTACATGCCATCAATTCCACTTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240604194334_rgi2 CTTACGAAGAAAGTGGAATTGATGGCATGTAG(+ACTCAC)GTACCAGAGTGCTTTAAAAAACATTATTACA NGG 10 8 17 8 24 0 100 5 10
    240604194333_l2fe ATATAATTTTAGTTTAGCAATAATTTTGACTG(+ACTCAC)GTACCAATAACTGTTTTTTAAAATTCCATAC NGG 10 8 17 8 24 0 100 5 10
    240604194332_m52m CATAAGTATGGAATTTTAAAAAACAGTTATTG(+ACTCAC)GTACCAGTCAAAATTATTGCTAAACTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604194330_50z7 TAATAACAGACCAGCGATTTTATAGAGGCCAG(+ACTCAC)GATCCTGAACTGGGGCATCCTCTCTGGAGTG NGG 10 8 17 8 24 0 100 5 10