Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194300_791n TCTTGAAGCTATACACCACCATCTAAGAAAGG(+ACTCAC)GATCCATATGCCAGTCAATCTTAGTGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240604194259_4515 ATTTTAAATTTTTTACCAAGCACATTCTGAAG(+ACTCAC)GTACCAACAAATGACATTATCTACTTCCTAC NGG 10 8 17 8 24 0 100 5 10
    240604194258_ob79 TACTAGTAGGAAGTAGATAATGTCATTTGTTG(+ACTCAC)GTACCTTCAGAATGTGCTTGGTAAAAAATTT NGG 10 8 17 8 24 0 100 5 10
    240604194257_a5cr TCTAAGAGTTCTGTGTATATTTCTTCTAGTTG(+ACTCAC)GTACCTACACAAATTAGATTTCTCTTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604194256_czjr TGGCAAAAGCAAGAGAAATCTAATTTGTGTAG(+ACTCAC)GTACCAACTAGAAGAAATATACACAGAACTC NGG 10 8 17 8 24 0 100 5 10
    240604194254_252v GTAAATTAACAACTCAGTTATTAAACTAGCAG(+ACTCAC)GATCCTACAATGATACATCTAGTGAAAGTAG NGG 10 8 17 8 24 0 100 5 10
    240604194253_0q2h TAACTCTACTTTCACTAGATGTATCATTGTAG(+ACTCAC)GATCCTGCTAGTTTAATAACTGAGTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240604194251_6ha9 ATTAGTTCTGATTCATCCCCGAGGGAAGAGAG(+ACTCAC)GTACCCATGGCTTCAGTGAGCACATGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604194249_5z0z CTGGGCTTGGCATGTGCTCACTGAAGCCATGG(+ACTCAC)GTACCTCTCTTCCCTCGGGGATGAATCAGAA NGG 10 8 17 8 24 0 100 5 10
    240604194248_secp TGGCAAGTGGCCAGCTGAGCCCAGGTTACTTG(+ACTCAC)GTACCCACTCTGCCTTGAGCCTTCCTTCCCC NGG 10 8 17 8 24 0 100 5 10