Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194247_5wif CCCTGGGGGAAGGAAGGCTCAAGGCAGAGTGG(+ACTCAC)GTACCAAGTAACCTGGGCTCAGCTGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240604194244_9h15 CCGCTGAGATCCAGCATCCAGCTGTGGCCAGG(+ACTCAC)GATCCACCACCTCTGTGTACACCCTCACCCG NGG 10 8 17 8 24 0 100 5 10
    240604194243_qk71 AGCAGCGGGTGAGGGTGTACACAGAGGTGGTG(+ACTCAC)GATCCCTGGCCACAGCTGGATGCTGGATCTC NGG 10 8 17 8 24 0 100 5 10
    240604194241_9eax GCTAAAAATTCTGCCTCTCTGTCACTGTGCTG(+ACTCAC)GATCCGTCTAAAGTGGGACAGTTCAATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604194240_g6et TTTCAGACCATTGAACTGTCCCACTTTAGACG(+ACTCAC)GATCCAGCACAGTGACAGAGAGGCAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240604194238_qwww ACCCAGTCTAAGCCCAGAGCTTGTTTGGGAGG(+ACTCAC)GATCCTCTCCTTCCTCCTGCTTGATCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604194237_nu3t GCTCCTACAGGATCAAGCAGGAGGAAGGAGAG(+ACTCAC)GATCCCTCCCAAACAAGCTCTGGGCTTAGAC NGG 10 8 17 8 24 0 100 5 10
    240604194235_cq0i CTTGATGTGTGAGGGGTAATACCCAGAGTGGG(+ACTCAC)GTACCTGCTTCACAAAGGATAAGGGGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240604194234_48ya ATTCCACCCTCCCCTTATCCTTTGTGAAGCAG(+ACTCAC)GTACCCCACTCTGGGTATTACCCCTCACACA NGG 10 8 17 8 24 0 100 5 10
    240604194232_wqr0 TGCCTGCCTGCCTGCCTGCCTGCCTACCTGTG(+ACTCAC)GATCCTGTTCCCCTAAATGGTCTGCCTTGTC NGG 10 8 17 8 24 0 100 5 10