Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194711_6k4z CTTTCCATCCTGAACCCATACTGCTGGATTTG(+ACTCAC)GATCCATGGACCTGAGGGCCATCAGCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604194708_ksgt CAGGAGTGGGGCTGATGGCCCTCAGGTCCATG(+ACTCAC)GATCCAAATCCAGCAGTATGGGTTCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240604194705_nefg CAATTTTAATGTTTCCTAAGCATTTTGACCAG(+ACTCAC)GTACCCAGGTTTAAGCTATGAACATTGACAG NGG 10 8 17 8 24 0 100 5 10
    240604194703_gfbf GGACACTGTCAATGTTCATAGCTTAAACCTGG(+ACTCAC)GTACCTGGTCAAAATGCTTAGGAAACATTAA NGG 10 8 17 8 24 0 100 5 10
    240604194701_n6sg CCTTGAGACCTTTATAAGCAAAAGCAAACCAG(+ACTCAC)GTACCCACCAGTTTGCTCAGACCCAGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240604194659_mz38 ACAAAGTCTGCTGGGTCTGAGCAAACTGGTGG(+ACTCAC)GTACCTGGTTTGCTTTTGCTTATAAAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240604194657_7bl7 AAAGTTAAAAATTCCTTAATTTTTTATTCCTG(+ACTCAC)GTACCACTACCACAATTTACAGGGCAATATA NGG 10 8 17 8 24 0 100 5 10
    240604194656_ohbs TCAGGTATATTGCCCTGTAAATTGTGGTAGTG(+ACTCAC)GTACCAGGAATAAAAAATTAAGGAATTTTTA NGG 10 8 17 8 24 0 100 5 10
    240604194655_4en3 CACTCAGGCCTGGAACTCGGTGCCCCCTCTCG(+ACTCAC)GATCCTGGCCTCACATGGGCCTCAGACCTAA NGG 10 8 17 8 24 0 100 5 10
    240604194653_6zet TTTTCTTAGGTCTGAGGCCCATGTGAGGCCAG(+ACTCAC)GATCCGAGAGGGGGCACCGAGTTCCAGGCCT NGG 10 8 17 8 24 0 100 5 10