Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194139_2j3o GCCTTCAGCGGGGGTGAAGAGCGCAGTGGAAG(+ACTCAC)GTACCCAGGAGGAGAAGAGGGCAAACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604194137_7y0l TCCTTGCATGTTTATGCTCTTATGTATTCCTG(+ACTCAC)GTACCCAGGGAGGCCAGAAGAGGGCTGATTC NGG 10 8 17 8 24 0 100 5 10
    240604194134_4iuk CCAGGGAATCAGCCCTCTTCTGGCCTCCCTGG(+ACTCAC)GTACCAGGAATACATAAGAGCATAAACATGC NGG 10 8 17 8 24 0 100 5 10
    240604194132_pqfu TGCTGCCGTGTATGTGCTGGGAATTGAACCAG(+ACTCAC)GATCCTCTGGATTTGCAGCTAGAACTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240604194131_j9r5 CATGGTTAAAAGTTCTAGCTGCAAATCCAGAG(+ACTCAC)GATCCTGGTTCAATTCCCAGCACATACACGG NGG 10 8 17 8 24 0 100 5 10
    240604194129_27fe CAGCTTACTGTTGCAGCTGCCTGAGCAGCCTG(+ACTCAC)GATCCTGCCTGAGGTTAGACCAGAGACCTCC NGG 10 8 17 8 24 0 100 5 10
    240604194127_2j1h GAGGAGGAGGTCTCTGGTCTAACCTCAGGCAG(+ACTCAC)GATCCAGGCTGCTCAGGCAGCTGCAACAGTA NGG 10 8 17 8 24 0 100 5 10
    240604194126_ds8n ACCCTAAAGTTTCTAGAAGGAGGCAGGGAAGG(+ACTCAC)GTACCCCAGGCTCCCCATCCCCAGGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604194123_vlpa GGACTCAGCTCCCTGGGGATGGGGAGCCTGGG(+ACTCAC)GTACCCTTCCCTGCCTCCTTCTAGAAACTTT NGG 10 8 17 8 24 0 100 5 10
    240604194122_tv89 AAACAGCTTCATGTTTGCCCCATTTCTGTCAG(+ACTCAC)GATCCCCCAACCATGGGGGGCACTTGCACAA NGG 10 8 17 8 24 0 100 5 10