Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194103_gap9 TGATTTGTGTCCCGCATCCCCTGGGGAAGCTG(+ACTCAC)GTACCATGGGCTTACTATGCTGTTTGGGACA NGG 10 8 17 8 24 0 100 5 10
    240604194101_g7ye GACGACCACTGGCTCCTAAAAGCACAGTCAGG(+ACTCAC)GATCCCTCTCAATGAGTGGGACCTAGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604194100_kkoq TGATGCCACCCTAGGTCCCACTCATTGAGAGG(+ACTCAC)GATCCCTGACTGTGCTTTTAGGAGCCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604194058_yqnn AGGGCAAGGCGAACATGAAAGTTAACATGCAG(+ACTCAC)GTACCTGGCAGAGTGGGGACCCCAGTGGATG NGG 10 8 17 8 24 0 100 5 10
    240604194057_7dun CCCAACATCCACTGGGGTCCCCACTCTGCCAG(+ACTCAC)GTACCTGCATGTTAACTTTCATGTTCGCCTT NGG 10 8 17 8 24 0 100 5 10
    240604194055_eect TGGTGATAAGCTGTGAGGAGTGAATGGACAGG(+ACTCAC)GATCCAGAGGGACACAGCAGGCTCCACAAAG NGG 10 8 17 8 24 0 100 5 10
    240604194053_juvu TTCCTCTTTGTGGAGCCTGCTGTGTCCCTCTG(+ACTCAC)GATCCCTGTCCATTCACTCCTCACAGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240604194051_skm7 GGAGAAACTGGGTTAAGGCCAGAGAAGTGCCG(+ACTCAC)GATCCTGCCCCAGATTTTGACTTTAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240604194049_lgff CATGGGAGCTCTAAAGTCAAAATCTGGGGCAG(+ACTCAC)GATCCGGCACTTCTCTGGCCTTAACCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240604194048_ygk0 GCCCGAGGTTCTGGTGGCCCAGCAACTGCAGG(+ACTCAC)GATCCATAAATCCAGAGGCCAGGATTCAGGC NGG 10 8 17 8 24 0 100 5 10