Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194006_c233 GCCACGTGGAAGACAGGATTTGGACATCTCTG(+ACTCAC)GATCCCAAGCCAAACACCATCTTGTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604194005_e4ks CTCCAGACCCATTTTTAGATAAGGGATTCAAG(+ACTCAC)GATCCAAGAAGACTTGTCCTGAATTCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240604194004_yl5j GCTAGCTCAGGAATTCAGGACAAGTCTTCTTG(+ACTCAC)GATCCTTGAATCCCTTATCTAAAAATGGGTC NGG 10 8 17 8 24 0 100 5 10
    240604194002_cbxd TGACCATTTCTGTAATTGGTACTGTCTGGCAG(+ACTCAC)GTACCATGTGTGGCCTGGCTGTCCTGGAACA NGG 10 8 17 8 24 0 100 5 10
    240604194001_6t9b TGCTGTGTTCCAGGACAGCCAGGCCACACATG(+ACTCAC)GTACCTGCCAGACAGTACCAATTACAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240604194000_olrq GGATGGACACAGGTCCCCCAGGGCCTAGGATG(+ACTCAC)GTACCTTGAGAAGACCAGCCTCATCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240604193958_zy8v GGGCCATGGATGATGAGGCTGGTCTTCTCAAG(+ACTCAC)GTACCATCCTAGGCCCTGGGGGACCTGTGTC NGG 10 8 17 8 24 0 100 5 10
    240604193957_2ncz AACTCAATTCTTGTTGGAAAAACAACAAAAAG(+ACTCAC)GTACCTAATCTAGTACAGCACCCTCAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604193956_osar CCTGGCTGGCTGAGGGTGCTGTACTAGATTAG(+ACTCAC)GTACCTTTTTGTTGTTTTTCCAACAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604193955_tivs TGTATGTAAAGTTCCAGGGATTTCAAAATCTG(+ACTCAC)GTACCTAATGTGGCAAATGCACGGCTGTGAG NGG 10 8 17 8 24 0 100 5 10