Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193919_qejl ACTTACAGACTAGAGTGGCCCTGGCCACACAG(+ACTCAC)GTACCTCTGCCCACCTGCCAGTCCAGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240604193918_rzkh AGGGAGAGGAGGAAGAGGGCAGCATCCAGAGG(+ACTCAC)GATCCCTATGGACCCCAGCACTGACTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240604193916_pfom TGGGTGCTGCAGTCAGTGCTGGGGTCCATAGG(+ACTCAC)GATCCCTCTGGATGCTGCCCTCTTCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240604193914_8fz7 CTTGATCCCAGGTGGATTCCCACCCTAACTGG(+ACTCAC)GTACCAGGACTCTGAACAGGCCAAGATGGGG NGG 10 8 17 8 24 0 100 5 10
    240604193912_ki1k GAGTGCCCCATCTTGGCCTGTTCAGAGTCCTG(+ACTCAC)GTACCCAGTTAGGGTGGGAATCCACCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604193910_054w GTGTCTCTTGGCACTGTCACCAAGTTGCCCAG(+ACTCAC)GATCCTGGGGATGCTGTAATTTGGAAAATTG NGG 10 8 17 8 24 0 100 5 10
    240604193907_27nr GCCCTCAATTTTCCAAATTACAGCATCCCCAG(+ACTCAC)GATCCTGGGCAACTTGGTGACAGTGCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240604193905_d0b3 CAGCAGCCACTGGGAAGAAGAGGCACAACAAG(+ACTCAC)GATCCATCCTCCCAGAGACCTTTCCAACTCC NGG 10 8 17 8 24 0 100 5 10
    240604193904_5csc TGGCAGGAGTTGGAAAGGTCTCTGGGAGGATG(+ACTCAC)GATCCTTGTTGTGCCTCTTCTTCCCAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240604193903_612y TCCTGGGCTTGGGCAGTGAGAGGAGCATCTAG(+ACTCAC)GTACCTGTTGTGCCCCTAATAAAGTGTTGGA NGG 10 8 17 8 24 0 100 5 10