Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193845_kk3w CTCTGGCCTTTGGAAGTCCAGTGTTCCCCAAG(+ACTCAC)GTACCCAGACCCAACTGGGTGCAGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240604193844_ijpi CCCAACCATGCAGGCTTACTCTCTGTGCTCTG(+ACTCAC)GTACCCGAGAAGGGGCCAAGTCTGTGTAGGG NGG 10 8 17 8 24 0 100 5 10
    240604193842_pe3i ACCCACCCTACACAGACTTGGCCCCTTCTCGG(+ACTCAC)GTACCAGAGCACAGAGAGTAAGCCTGCATGG NGG 10 8 17 8 24 0 100 5 10
    240604193841_k4t4 TAGCTAGCTGGATGCTGTTTACTGGGTGAAAG(+ACTCAC)GTACCTTGGGAGTTTTCATGGCTCCAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604193838_wx12 CATCCCTGCTTGGAGCCATGAAAACTCCCAAG(+ACTCAC)GTACCTTTCACCCAGTAAACAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240604193836_q17h TGGCCCTACAGAACACACCTGAGCCTCTTGGG(+ACTCAC)GTACCATCGCAGGGTAGCCCTCCCTAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240604193835_ji7m AACTAGGCCCTAGGGAGGGCTACCCTGCGATG(+ACTCAC)GTACCCCAAGAGGCTCAGGTGTGTTCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240604193833_z7qu CCAAGAACCAACTAGCTGCCTCAGGTCTCGTG(+ACTCAC)GATCCCAGATGGACATGGCAGTAACTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604193832_5yc8 ACCGGCTCTGAGTTACTGCCATGTCCATCTGG(+ACTCAC)GATCCACGAGACCTGAGGCAGCTAGTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240604193829_fn7j TCTCACCTGAGAAATGGTGCAAACCAACCAGG(+ACTCAC)GATCCTTAGGCTACTGAGAAGGCTGTCTGCT NGG 10 8 17 8 24 0 100 5 10