Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604194204_q0sy GAAGAAGGACCGAGGGAGTTAAGTATCGGATG(+ACTCAC)GTACCAAGGGTCAGACTCTTGAGGTCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240604194202_b9yl TTCTTCCTCTTCCTCTTCCTCCTTGAATCTGG(+ACTCAC)GTACCCTAACCCTGAGAAGAAAACCCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240604194200_08of AGAAAACTGAGGGTTTTCTTCTCAGGGTTAGG(+ACTCAC)GTACCCAGATTCAAGGAGGAAGAGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604194158_zgsm GCACAGCACCTACCAGCCCTGGCCCTTCCTTG(+ACTCAC)GTACCATCTCCACCACTGCTGGACTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240604194157_7w2g GGGTAGGTGGGAGTCCAGCAGTGGTGGAGATG(+ACTCAC)GTACCAAGGAAGGGCCAGGGCTGGTAGGTGC NGG 10 8 17 8 24 0 100 5 10
    240604194156_kzgv ATATGATGAGCCCAAGAGATCTGGGATGAATG(+ACTCAC)GATCCCTAGATACGGTAGGCCCAGATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240604194155_7ozh GGTGGGGGAGATCTGGGCCTACCGTATCTAGG(+ACTCAC)GATCCATTCATCCCAGATCTCTTGGGCTCAT NGG 10 8 17 8 24 0 100 5 10
    240604194153_yde6 GCCACCCCACAGCTGGGGCTTGGAGGCTTCTG(+ACTCAC)GATCCAGGGGGTTGTGGCTTGCATTGCATCT NGG 10 8 17 8 24 0 100 5 10
    240604194150_8ccx GGTACAGATGCAATGCAAGCCACAACCCCCTG(+ACTCAC)GATCCAGAAGCCTCCAAGCCCCAGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240604194149_orzc GGGGAAACCCAGGGAAGTTATATAAACATTGG(+ACTCAC)GTACCACTGTGTCATTGCAGTTCTGTGGCCC NGG 10 8 17 8 24 0 100 5 10