Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193703_9fxz TGAACCTGGAGGTGCCAAAGAAGCCAGCAATG(+ACTCAC)GATCCAGAGCAGCTGCCATAAGACCTCACAG NGG 10 8 17 8 24 0 100 5 10
    240604193701_ew3z GGTTTGTCCAAATGTGTGGCATGGGAGATAGG(+ACTCAC)GTACCCCTGAAAGCAAGGAAAGGAGCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240604193659_yeaq CCACAGCTGGGCTCCTTTCCTTGCTTTCAGGG(+ACTCAC)GTACCCTATCTCCCATGCCACACATTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240604193657_s8h1 TACTGCCTTCCTGGGTTCCATGCCAGATGTTG(+ACTCAC)GATCCTAAGCAGGAGGTAGCTGAGTTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240604193656_2b1s GGTCCATCTCAACTCAGCTACCTCCTGCTTAG(+ACTCAC)GATCCAACATCTGGCATGGAACCCAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604193654_ogu6 AATGCTTATGAATATTTGTGATCTATAGGTTG(+ACTCAC)GATCCATAGCAACACCAACACACAAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240604193653_4ypk GATCCCATTTTTTGTGTGTTGGTGTTGCTATG(+ACTCAC)GATCCAACCTATAGATCACAAATATTCATAA NGG 10 8 17 8 24 0 100 5 10
    240604193652_lsxx AAAAAGAAGCTTACAATAATGACCTAAGTATG(+ACTCAC)GATCCAGTGGGGTATTCCACCACACAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240604193650_8zhr ACCTTTGCACTGTGTGGTGGAATACCCCACTG(+ACTCAC)GATCCATACTTAGGTCATTATTGTAAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240604193649_0rmt TGTCTTCCATGCCTCCTGGTGAATCTTGCTTG(+ACTCAC)GTACCATTCAATCCCATGTGTAAAGACAGAC NGG 10 8 17 8 24 0 100 5 10