Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193612_h6db AAAAGGAAACATTTCTGTAACACTGGAACTAG(+ACTCAC)GATCCTGGGCCTTGGAGGGGTTAGCTACTAC NGG 10 8 17 8 24 0 100 5 10
    240604193609_ydg6 CACTTTACTGTTTCAATGGTAATAATGCACCG(+ACTCAC)GTACCTCAATTCAACTGCTACAAATAAATGT NGG 10 8 17 8 24 0 100 5 10
    240604193608_atkn TTTTGACATTTATTTGTAGCAGTTGAATTGAG(+ACTCAC)GTACCGGTGCATTATTACCATTGAAACAGTA NGG 10 8 17 8 24 0 100 5 10
    240604193606_t02b ATGTATCCTTACTTTGACAATTAGACACACAG(+ACTCAC)GTACCAGGAAAAAAGGAATTGACTTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240604193604_41wl TTCCAGCCTGAAAGTCAATTCCTTTTTTCCTG(+ACTCAC)GTACCTGTGTGTCTAATTGTCAAAGTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604193602_tpp2 TGGTTTGCATTGACCGCTTTCCTCCTTGACTG(+ACTCAC)GTACCTTTCCCGTCTTTACGTATGGGAATTG NGG 10 8 17 8 24 0 100 5 10
    240604193559_yns5 GAGGGCAATTCCCATACGTAAAGACGGGAAAG(+ACTCAC)GTACCAGTCAAGGAGGAAAGCGGTCAATGCA NGG 10 8 17 8 24 0 100 5 10
    240604193557_ylmy GAGCGCATCCCAGCATGGAGCAGGTAGAGTGG(+ACTCAC)GTACCAAGGCACACCCCTAGCTCAGAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240604193555_qy7a GCCAATAGCCTCTGAGCTAGGGGTGTGCCTTG(+ACTCAC)GTACCCACTCTACCTGCTCCATGCTGGGATG NGG 10 8 17 8 24 0 100 5 10
    240604193553_wc1m CGAGAGGTTTTCACAAACCGAGTCAAAACAAG(+ACTCAC)GTACCAGTTTTGAGCTCACATCAACTTTTTA NGG 10 8 17 8 24 0 100 5 10