Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604193103_s8rc GGATGACAGAACCATATCAGTGCAAACCCCAG(+ACTCAC)GATCCAAGGCTGCAAGCCTAAGTCACAAGTC NGG 10 8 17 8 24 0 100 5 10
    240604193101_acop CATAGCATTCACCATGATCATATGCCCCTCGG(+ACTCAC)GATCCTGAGAGAAGAGAGAGGCTTGTACTGA NGG 10 8 17 8 24 0 100 5 10
    240604193100_o1le ACCCCTCAGTACAAGCCTCTCTCTTCTCTCAG(+ACTCAC)GATCCCGAGGGGCATATGATCATGGTGAATG NGG 10 8 17 8 24 0 100 5 10
    240604193058_67sw GATAGACATTATCTCCCCCCCCCCTTTTTCAG(+ACTCAC)GTACCTGTAATTTGTGTCTGAGAGGCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240604193057_6ym7 TTCCCACCCTGCCTCTCAGACACAAATTACAG(+ACTCAC)GTACCTGAAAAAGGGGGGGGGGAGATAATGT NGG 10 8 17 8 24 0 100 5 10
    240604193056_jlw6 GGTGTCATCGTTTGGAGGCTGATTATGGGATG(+ACTCAC)GATCCCCGGGTATGGCAGTCTCAGAATACTT NGG 10 8 17 8 24 0 100 5 10
    240604193052_2xo0 GATAAAAGTATTCTGAGACTGCCATACCCGGG(+ACTCAC)GATCCATCCCATAATCAGCCTCCAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240604193051_a76g AGGTATTCCCCGGTCCCTACAGCTAATCCATG(+ACTCAC)GATCCCTGAAAAGGGACTGCAGGCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    240604193049_vavt CAGTACTGTCCTGCCTGCAGTCCCTTTTCAGG(+ACTCAC)GATCCATGGATTAGCTGTAGGGACCGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604193048_3fya TTTCATAACTTCAATAGCTTTTTTCTTTTCGG(+ACTCAC)GATCCCAGTGTTGGCTCTATTTCACTTTCTA NGG 10 8 17 8 24 0 100 5 10