Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192959_kgaa TGATTTATGTGTCCCTCTATGTATCTGAGGTG(+ACTCAC)GTACCAACAACAGTAGCGGCAGCAGTAGCTA NGG 10 8 17 8 24 0 100 5 10
    240604192957_nlzb CGTCTGCTTCCCTCTCTCCTCGTGCTAAGCTG(+ACTCAC)GATCCGTGTCCTTTTCTTTTTAACGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240604192956_5vuq TCACTTGAACTCGTTAAAAAGAAAAGGACACG(+ACTCAC)GATCCAGCTTAGCACGAGGAGAGAGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240604192954_2grw GCTCACCCGCAAGGGGTTCCAGAACAAATCTG(+ACTCAC)GTACCCACGAGGACAAGCCAAACCAAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604192953_dw25 CCTGATCCACTTGGTTTGGCTTGTCCTCGTGG(+ACTCAC)GTACCAGATTTGTTCTGGAACCCCTTGCGGG NGG 10 8 17 8 24 0 100 5 10
    240604192951_gk5x AAGAGAAGTAGGAGGTTGACTGACCAACTTGG(+ACTCAC)GTACCATAGGATTTTGTTTATTGCATAGGTC NGG 10 8 17 8 24 0 100 5 10
    240604192949_u27x TAGATGACCTATGCAATAAACAAAATCCTATG(+ACTCAC)GTACCCAAGTTGGTCAGTCAACCTCCTACTT NGG 10 8 17 8 24 0 100 5 10
    240604192946_t293 GCTATGCACCCCTCTCCCTGGCACTGACCCTG(+ACTCAC)GATCCCCATCTTCTGTACCCAGTGACTAGAC NGG 10 8 17 8 24 0 100 5 10
    240604192944_5i2t CAGTCGTCTAGTCACTGGGTACAGAAGATGGG(+ACTCAC)GATCCAGGGTCAGTGCCAGGGAGAGGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240604192942_kt5g TTCCTGCCAAACTACCTTGCGAGGTTTCCTGG(+ACTCAC)GTACCCAAGTGGAGCCAAAGCTCACTAGGAC NGG 10 8 17 8 24 0 100 5 10