Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192921_wtuv TTAACCTCCCTTGTTTCTTTTGGGGGTGGGGG(+ACTCAC)GTACCAATAGGCCAGAGAGAAGGCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604192920_7rxv TGGGGGCTCTTAAGGATTTCCCAGAAAAACAG(+ACTCAC)GATCCTGGGATGGGACGTGGTTGCTCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240604192918_pikt TTTTTTGCCCGAGCAACCACGTCCCATCCCAG(+ACTCAC)GATCCTGTTTTTCTGGGAAATCCTTAAGAGC NGG 10 8 17 8 24 0 100 5 10
    240604192914_1dje ACCACACATACCTTGAGGAGGACTGCCCTAGG(+ACTCAC)GTACCAGGTTTTATTCAGCTCTAAGTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604192913_peqz TTACTCTGGGACTTAGAGCTGAATAAAACCTG(+ACTCAC)GTACCCTAGGGCAGTCCTCCTCAAGGTATGT NGG 10 8 17 8 24 0 100 5 10
    240604192911_0b10 TATGTGTGGGGATCCCTTCACCCACCATACTG(+ACTCAC)GTACCCCTTCTTCAGCTCCATCTGTCCCTAT NGG 10 8 17 8 24 0 100 5 10
    240604192909_3p35 AGGGGATAGGGACAGATGGAGCTGAAGAAGGG(+ACTCAC)GTACCAGTATGGTGGGTGAAGGGATCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604192907_jr6r CTTCTTCTTTCTCTAGTGCAAGTATGTGTGGG(+ACTCAC)GATCCCTTCACCCACCATACTGGTACCCCTT NGG 10 8 17 8 24 0 100 5 10
    240604192906_rowe TGAAGAAGGGGTACCAGTATGGTGGGTGAAGG(+ACTCAC)GATCCCCACACATACTTGCACTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604192903_3pq1 TATTTGCTGTGCAAAGCACGCCCTAAGATGGG(+ACTCAC)GTACCTTTGATCTCACCGTGCGGCTGCAGGA NGG 10 8 17 8 24 0 100 5 10