Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192940_q7xl GTCCTGTCCTAGTGAGCTTTGGCTCCACTTGG(+ACTCAC)GTACCCAGGAAACCTCGCAAGGTAGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240604192938_5wat CATGGAGAGACTTAATAAAGGGTTTTACAAGG(+ACTCAC)GTACCTGGATCCCAGGGGGAAGTAGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240604192936_1wgc CCAAGGATAAACTACTTCCCCCTGGGATCCAG(+ACTCAC)GTACCCTTGTAAAACCCTTTATTAAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604192934_51tj AGACTTAATAAAGGGTTTTACAAGGGTACCTG(+ACTCAC)GATCCCAGGGGGAAGTAGTTTATCCTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604192931_aeof CTGTGCCCCAAGGATAAACTACTTCCCCCTGG(+ACTCAC)GATCCAGGTACCCTTGTAAAACCCTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240604192929_h81x TCTCAAAGATGAGGCCCACATGAACCTGCTGG(+ACTCAC)GATCCCACGAACACATATTGGACCTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604192928_48wv TCCCTGTGCTCAGGTCCAATATGTGTTCGTGG(+ACTCAC)GATCCCAGCAGGTTCATGTGGGCCTCATCTT NGG 10 8 17 8 24 0 100 5 10
    240604192926_5pou ACCACATGATAGCTCGCAACCATCTATAATAG(+ACTCAC)GATCCAAACACTCTTCTGGTGTGTCTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240604192925_bzs7 AGCTGTCTTCAGACACACCAGAAGAGTGTTTG(+ACTCAC)GATCCTATTATAGATGGTTGCGAGCTATCAT NGG 10 8 17 8 24 0 100 5 10
    240604192924_gekh GTGGCATGCCCAGCCTTCTCTCTGGCCTATTG(+ACTCAC)GTACCCCCCACCCCCAAAAGAAACAAGGGAG NGG 10 8 17 8 24 0 100 5 10